A homology independent sequence replacement strategy in human cells using a CRISPR nuclease.
Danner E, Lebedin M, de la Rosa K, Kuhn R
Open Biol. 2021 Jan;11(1):200283. doi: 10.1098/rsob.200283. Epub 2021 Jan 27.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 149344 | pAAVS1-NEO-CAG-BFP-pA-GFP-pA | Vector for targeting the Replace reporter to the AAVS1 locus |
| 149345 | pReplace_donor_mcherry | Donor vector for the replacement of reporter BFP by mcherry |
| 149346 | pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26 | Mammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNA |
| 149347 | pCbh-SpyCas9-2A-puro-pA_U6-BbsI | Mammalian expression vector for SpyCas9 and puromycin resistance |
| 149348 | pMC_cherry donor_Rosa target (pE002) | Replace donor vector for cherry insertion using the mouse Rosa26 guide RNA |
| 149349 | pMC_cherry donor_Polb target (pE038) | Replace donor vector for cherry insertion using the human Polb guide RNA |
| 149350 | pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047) | Mammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNA |
| 149351 | pCAG-Venus-pA_U6-BbsI (pE057) | Mammalian expression vector for Venus |
| 149352 | pCAG-BFP-pA_U6-BbsI (pE058) | Mammalian expression vector for BFP |
| 149353 | pCAG-miRFP670-pA_U6-BbsI (pE059) | Mammalian expression vector for miRFP670 |
| 149354 | pCMV-UnaG-pA (pE091) | Mammalian Expression vector for UnaG |