Sources of off-target expression from recombinase-dependent AAV vectors and mitigation with cross-over insensitive ATG-out vectors.
Fischer KB, Collins HK, Callaway EM
Proc Natl Acad Sci U S A. 2019 Dec 16. pii: 1915974116. doi: 10.1073/pnas.1915974116.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 149294 | pAAV-nef-CIAO-Flp | Cre-dependent expression of Flp recombinase. |
| 149295 | pAAV-nef-CIAO-TVA-mCherry | Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing. |
| 149296 | pAAV-nef-CIAO2-Flp | Cre-dependent expression of Flp recombinase. |
| 149297 | pAAV-nef-CIAO2-TVA-mCherry | Cre-dependent expression of TVA:mCherry fusion protein for rabies tracing. |