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Distinct modes of recruitment of the CCR4-NOT complex by Drosophila and vertebrate Nanos.

Raisch T, Bhandari D, Sabath K, Helms S, Valkov E, Weichenrieder O, Izaurralde E
EMBO J. 2016 May 2;35(9):974-90. doi: 10.15252/embj.201593634. Epub 2016 Mar 11. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
147818pAc5.1C-Fluc-DmHunchback_WInsect Expression of DmHunchback-3UTR
148327pnEA-NpG-DmNanos_116-163_ABBacterial Expression of DmNanos_116-163
148328pnEA-NpG-DmNanos_116-163-E151AF152AN155A_ABBacterial Expression of DmNanos_116-163-E151AF152AN155A
148329pnEA-NpG-DmNanos_116-163-I123M_ABBacterial Expression of DmNanos_116-163-I123M
148330pnEA-NpG-DmNanos-opt_50-236-del116-163-GB1opt_ABBacterial Expression of DmNanos-opt_50-236-del116-163
148331pnEA-NpG-DmNanos-opt_50-236-GB1opt_ABBacterial Expression of DmNanos-opt_50-236
148332pnEA-NpG-DmNanos-opt_116-163-F152E_ABBacterial Expression of DmNanos-opt_116-163-F152E
148333pnEA-NpG-DmNanos-opt_116-163-L127DF130D_ABBacterial Expression of DmNanos-opt_116-163-L127DF130D
148334pnEA-NpH-HsNot3_607-748-Y702A-NpM-HsNot2_350-540_ABBacterial Expression of HsNot3_607-748-Y702A-HsNot2_350-540
148335pnYC-NpM-HsNot1iso1_1833-2361-H1949D_ABBacterial Expression of HsNot1iso1_1833-2361-H1949D
148336pnYC-NpM-HsNot1iso1_1833-2361-V1880E_ABBacterial Expression of HsNot1iso1_1833-2361-V1880E
148455pAc5.1B-EGFP-DmNanos_ACInsect Expression of DmNanos
148456pAc5.1B-EGFP-DmNanos_1-296_ACInsect Expression of DmNanos_1-296
148457pAc5.1B-EGFP-DmNanos_50-236_ACInsect Expression of DmNanos_50-236
148458pAc5.1B-EGFP-DmNanos_297-381_ACInsect Expression of DmNanos_297-381
148459pAc5.1B-EGFP-DmNanos-del50-236_ACInsect Expression of DmNanos-del50-236
148460pAc5.1B-lambdaN-HA-DmNanos_ACInsect Expression of DmNanos
148461pAc5.1B-lambdaN-HA-DmNanos_1-296_ACInsect Expression of DmNanos_1-296
148462pAc5.1B-lambdaN-HA-DmNanos_del 50-236_ACInsect Expression of DmNanos-del50-236
148463pAc5.1B-lambdaN-HA-DmNot1_1710-2505-V2002E-dsRNAres_ACInsect Expression of DmNot1_1710-2505-V200E-dsRNAres
148464pAc5.1B-lambdaN-HA-GST-DmNanos_50-115_ACInsect Expression of DmNanos_50-115
148465pAc5.1B-lambdaN-HA-GST-DmNanos_50-236_ACInsect Expression of DmNanos_50-236
148466pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-E151AF152AN155A-V5His6_ACInsect Expression of DmNanos_116-163-E151AF152AN1555A
148467pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-F152E-V5His6_ACInsect Expression of DmNanos_116-163-F152E
148468pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-L127DF130D-V5His6_ACInsect Expression of DmNanos_116-163-L127DF130D
148469pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-V5His6_ACInsect Expression of DmNanos_116-163
148470pAc5.1B-lambdaN-HA-GST-DmNanos_164-236_ACInsect Expression of DmNanos_164-236
148471pAc5.1B-lambdaN-HA-GST-DmNanos_297-381_ACInsect Expression of DmNanos_297-381
148472pAc5.1B-lambdaN-HA-GST-HsNanos2_6-22-DmNanos_297-381_ACInsect Expression of HsNanos2_6-22-DmNanos_297-381
148473pAc5.1B-lambdaN-HA-GST-HsNanos2_6-22-F6AW9E-DmNanos_297-381_ACInsect Expression of HsNanos2_6-22-F6AW9E-DmNanos_297-381
148500pAc5.1B-lambdaN-HA-DmNot1_1710-2505-H2067D-dsRNAres_ADInsect Expression of DmNot1_1710-2505-H2067D-dsRNAres
148501pAc5.1C-Fluc-DmHunchback-delBoxA_ADInsect Expression of DmHunchback-delBoxA
148502pAc5.1C-Fluc-DmHunchback-delBoxB_ADInsect Expression of DmHunchback-delBoxB

Antibodies from Article