GIGYF1/2 proteins use auxiliary sequences to selectively bind to 4EHP and repress target mRNA expression.
Peter D, Weber R, Sandmeir F, Wohlbold L, Helms S, Bawankar P, Valkov E, Igreja C, Izaurralde E
Genes Dev. 2017 Jun 1;31(11):1147-1161. doi: 10.1101/gad.299420.117. Epub 2017 Jul 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 148144 | pT7-V5-SBP-C1-Hs4EBP1-L75AV81A_Z | Mammalian Expression of Hs4EBP1-L75AV81A |
| 148145 | pT7-V5-SBP-C1-Hs4EBP1-Y54AL59A_Z | Mammalian Expression of Hs4EBP1-Y54AL59A |
| 148146 | pT7-V5-SBP-C1-Hs4EBP1-YLLVtoAAAA_Z | Mammalian Expression of Hs4EBP1-YLLVtoAAAA |
| 148147 | pT7-V5-SBP-C1-Hs4EBP3-L61AV67A_Z | Mammalian Expression of Hs4EBP3-L61AV67A |
| 148148 | pT7-V5-SBP-C1-Hs4EBP3-Y40AL45A_Z | Mammalian Expression of Hs4EBP3-Y40AL45A |
| 148149 | pT7-V5-SBP-C1-Hs4EBP3-YLLVtoAAAA_Z | Mammalian Expression of Hs4EBP3-YLLVtoAAAA |
| 148549 | pLNHA-C1-Hs4EHP_AE | Mammalian Expression of Hs4EHP |
| 148550 | pLNHA-C1-HsGIGYF2_AE | Mammalian Expression of HsGIGYF2 |
| 148551 | pLNHA-Hs4EHP-S99N_AE | Mammalian Expression of Hs4EHP-S99N |
| 148552 | pnEA-NpM-HsGIGYF1_33-52-GB1_AE | Bacterial Expression of HsGIGYF1_33-52 |
| 148553 | pnEA-NpM-HsGIGYF1_33-71_AE | Bacterial Expression of HsGIGYF1_33-71 |
| 148554 | pnEA-NpM-HsGIGYF1_33-71-GB1_AE | Bacterial Expression of HsGIGYF1_33-71 |
| 148555 | pnEA-NpM-HsGIGYF1_33-103-E44AE45FQ87A_AE | Bacterial Expression of HsGIGYF1_33-103-E44AE45FQ87A |
| 148556 | pnEA-NpM-HsGIGYF1_33-103-E44AE45FQ87A-GB1_AE | Bacterial Expression of HsGIGYF1_33-103-E44AE45FQ87A |
| 148557 | pnEA-NpM-HsGIGYF1_33-103-GB1_AE | Bacterial Expression of HsGIGYF1_33-103 |
| 148558 | pnEA-NpM-HsGIGYF2_35-54-GB1_AE | Bacterial Expression of HsGIGYF2_35-54 |
| 148559 | pnEA-NpM-HsGIGYF2_35-72-GB1_AE | Bacterial Expression of HsGIGYF2_35-72 |
| 148560 | pnEA-NpM-HsGIGYF2_35-105-GB1_AE | Bacterial Expression of HsGIGYF2_35-105 |
| 148561 | pnYC-CvH-Hs4EHP_1-234_AE | Bacterial Expression of Hs4EHP_1-234 |
| 148562 | pnYC-CvH-Hs4EHP_1-234-I85AM101A_AE | Bacterial Expression of Hs4EHP_1-234-I85AM101A |
| 148563 | pnYC-CvH-Hs4EHP_1-234-W95A_AE | Bacterial Expression of Hs4EHP_1-234-W95A |
| 148564 | pnYC-CvH-Hs4EHP_52-234opt_AE | Bacterial Expression of Hs4EHP_52-234opt |
| 148565 | pnYC-CvH-Hs4EHP_52-234opt-R103LE149L_AE | Bacterial Expression of Hs4EHP_52-234opt-R103LE149L |
| 148566 | pnYC-NpH-Hs4EHP_52-234opt_AE | Bacterial Expression of Hs4EHP_52-234opt |
| 148567 | pnYC-NpH-Hs4EHP_52-234opt-Q159SM161DR202E_AE | Bacterial Expression of Hs4EHP_52-234opt-Q159SM161DR202E |
| 148569 | pT7-EGFP-C1-HsGIGYF1_AE | Mammalian Expression of HsGIGYF1 |
| 148570 | pT7-EGFP-C1-HsGIGYF1_1-177_AE | Mammalian Expression of HsGIGYF1_1-177 |
| 148571 | pT7-EGFP-C1-HsGIGYF1_1-177-E86AN95F_AE | Mammalian Expression of HsGIGYF1_1-177-E86AN95F |
| 148572 | pT7-EGFP-C1-HsGIGYF1_1-177-L60DF65DV68D_AE | Mammalian Expression of HsGIGYF1_1-177-L60DF65DV68D |
| 148573 | pT7-EGFP-C1-HsGIGYF1_1-177-LFVPLtoDDDDA_AE | Mammalian Expression of HsGIGYF1_1-177-LFVPLtoDDDDA |
| 148574 | pT7-EGFP-C1-HsGIGYF1_1-177-P76DL77A_AE | Mammalian Expression of HsGIGYF1_1-177-P76DL77A |
| 148575 | pT7-EGFP-C1-HsGIGYF1_1-177-YYMLtoAAAA_AE | Mammalian Expression of HsGIGYF1_1-177-YYMLtoAAAA |
| 148576 | pT7-EGFP-C1-HsGIGYF1-Y39AY41AM46AL47A_AE | Mammalian Expression of HsGIGYF1-Y39AY41AM46AL47A |
| 148577 | pT7-EGFP-C1-HsGIGYF2_AE | Mammalian Expression of HsGIGYF2 |
| 148578 | pT7-EGFP-C1-HsGIGYF2_1-180_AE | Mammalian Expression of HsGIGYF2_1-180 |
| 148579 | pT7-EGFP-C1-HsGIGYF2_1-180-P78DL79A_AE | Mammalian Expression of HsGIGYF2_1-180-P78DL79A |
| 148580 | pT7-EGFP-C1-HsGIGYF2_1-180-YYMLtoAAAA_AE | Mammalian Expression of HsGIGYF2_1-180-YYMLtoAAAA |
| 148581 | pT7-EGFP-HsGIGYF1_1-177-LFVENtoDDDAF_AE | Mammalian Expression of HsGIGYF1_1-177-LFVENtoDDDAF |
| 148582 | pT7-EGFP-HsGIGYF2_1-180-LFItoDDD_AE | Mammalian Expression of HsGIGYF2_1-180-LFItoDDD |
| 148583 | pT7-V5-SBP-C1-Hs4EHP-I85AM101A_AE | Mammalian Expression of Hs4EHP-I85AM101A |
| 148584 | pT7-V5-SBP-C1-Hs4EHP-I85AW95AM101A_AE | Mammalian Expression of Hs4EHP-I85AW95AM101A |
| 148585 | pT7-V5-SBP-C1-Hs4EHP-R103LE149L_AE | Mammalian Expression of Hs4EHP-R103LE149L |
| 148587 | pT7-V5-SBP-C1-Hs4EHP-W95AR103LE149L_AE | Mammalian Expression of Hs4EHP-W95AR103LE149L |
| 148588 | pT7-V5-SBP-Hs4EHP_AE | Mammalian Expression of Hs4EHP |
| 148686 | pnEA-NpM-HsGIGYF2_35-105-E46AE47FN91A-GB1_AF | Bacterial Expression of HsGIGYF2_35-105-E46AE47FN91A |
| 148687 | pT7-EGFP-C1-Hs4E-T_AF | Mammalian Expression of Hs4E-T |
| 148688 | pT7-EGFP-C1-HsGIGYF2_1-180-E88AN97F_AF | Mammalian Expression of HsGIGYF2_1-180-E88AN97F |
| 148689 | pT7-EGFP-C1-HsGIGYF2_1-180-LFIENtoDDDAF_AF | Mammalian Expression of HsGIGYF2_1-180-LFIENtoDDDAF |
| 148690 | pT7-EGFP-C1-HsGIGYF2_1-180-LFIPLtoDDDDA_AF | Mammalian Expression of HsGIGYF2_1-180-LFIPLtoDDDDA |
| 148691 | pT7-EGFP-C1-HsGIGYF2-YYMLtoAAAA_AF | Mammalian Expression of HsGIGYF2-YYMLtoAAAA |
| 148692 | pT7-V5-SBP-C1-Hs4EHP-S99N_AF | Mammalian Expression of Hs4EHP-S99N |
| 148878 | pCIneo-luc-TNFalpha-ARE-polyA-Malat_AH | Mammalian Expression of TNFalpha-ARE-polyA-Malat |
| 148879 | pLNHA-C1-HsTTP_1-313_AH | Mammalian Expression of HsTTP_1-313 |