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Structure of the Dcp2-Dcp1 mRNA-decapping complex in the activated conformation.

Valkov E, Muthukumar S, Chang CT, Jonas S, Weichenrieder O, Izaurralde E
Nat Struct Mol Biol. 2016 Jun;23(6):574-9. doi: 10.1038/nsmb.3232. Epub 2016 May 16. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
148003pnEA-NpG-SpDCP1_XBacterial Expression of SpDCP1
148004pnEA-NpH-CtDCP2_1-267_XBacterial Expression of CtDCP2_1-267
148005pnEA-NpM-SpEDC3_1-94_XBacterial Expression of SpEDC3_1-84
148006pnYC-NpM-CtDCP1_XBacterial Expression of CtDCP1
148007pnYC-NpM-SpDcp2_1-266_XBacterial Expression of SpDCP2_1-266
148008pnYC-NpM-SpDCP2_1-242_XBacterial Expression of SpDCP2_1-242
148009pnYC-NpM-SpDCP2_1-253_XBacterial Expression of SpDCP2_1-253
148538pnEA-NpG-SpDcp1opt_ADBacterial Expression of SpDcp1opt
148539pnEA-NpG-SpDcp1opt-K44A_ADBacterial Expression of SpDcp1opt-K44A
148540pnEA-NpG-SpDcp1opt-K44AK47A_ADBacterial Expression of SpDcp1opt-K44AK47A
148541pnEA-NpG-SpDcp1opt-W107A_ADBacterial Expression of SpDcp1opt-W107A
148542pnEA-NpG-SpDcp1opt-Y36A_ADBacterial Expression of SpDcp1opt-Y36A
148543pnEA-NpG-SpScp1opt-K47A_ADBacterial Expression of SpDcp1opt-K47A
148544pnEA-NvM-SpEdc1opt_155-180-GB1_ADBacterial Expression of SpEDC1opt_155-180
148545pnYC-NpM-SpDcp2opt_1-242_ADBacterial Expression of SpDcp2opt_1-242
148546pnYC-NpM-SpDcp2opt_1-242-E147Q_ADBacterial Expression of SpDcp2opt_1-242-E147Q
148547pnYC-SpDcp2opt_1-242_ADBacterial Expression of SpDcp2opt_1-242

Antibodies from Article