A CAF40-binding motif facilitates recruitment of the CCR4-NOT complex to mRNAs targeted by Drosophila Roquin.
Sgromo A, Raisch T, Bawankar P, Bhandari D, Chen Y, Kuzuoglu-Ozturk D, Weichenrieder O, Izaurralde E
Nat Commun. 2017 Feb 6;8:14307. doi: 10.1038/ncomms14307.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 147909 | pnEA-NpH-HsNot3_607-748-NpM-HsNot2_350-540_W | Bacterial Expression of HsNot3_607-748-HsNot2_350-540 |
| 148525 | pAc5.1B-EGFP-DmRoquin_AD | Insect Expression of DmRoquin |
| 148526 | pAc5.1B-lambdaN-HA-DmRoquin_AD | Insect Expression of DmRoquin |
| 148527 | pAc5.1B-lambdaN-HA-DmRoquin_1-500_AD | Insect Expression of DmRoquin_1-500 |
| 148528 | pAc5.1B-lambdaN-HA-DmRoquin_501-819_AD | Insect Expression of DmRoquin_501-819 |
| 148529 | pAc5.1B-lambdaN-HA-EGFP-DmRoquin_501-702_AD | Insect Expression of DmRoquin_501-702 |
| 148530 | pAc5.1B-lambdaN-HA-GST-DmRoquin_501-702_AD | Insect Expression of DmRoquin_501-702 |
| 148531 | pcDNA3.1-MS2-HA-DmRoquin_AD | Mammalian Expression of DmRoquin |
| 148532 | pcDNA3.1-MS2-HA-DmRoquin_1-500_AD | Mammalian Expression of DmRoquin_1-500 |
| 148533 | pcDNA3.1-MS2-HA-DmRoquin_501-819_AD | Mammalian Expression of DmRoquin_501-819 |
| 148534 | pnYC-NpM-DmRoquin_501-819-GB1-His6_AD | Bacterial Expression of DmRoquin_501-819 |
| 148535 | pT7-V5-SBP-C1-DmRoquin_AD | Mammalian Expression of DmRoquin |
| 148536 | pT7-V5-SBP-C1-DmRoquin_1-500_AD | Mammalian Expression of DmRoquin_1-500 |
| 148537 | pT7-V5-SBP-C1-DmRoquin_501-819_AD | Mammalian Expression of DmRoquin_501-819 |
| 148589 | pAc5.1B-EGFP-DmCaf40-Y139DG146E_AE | Insect Expression of DmCaf40-Y139DG146E |
| 148590 | pAc5.1B-EGFP-DmRoquin-del725-755_AE | Insect Expression of DmRoquin-del725-755 |
| 148591 | pAc5.1B-EGFP-DmRoquin-del725-755-del790-812_AE | Insect Expression of DmRoquin-del725-755-del790-812 |
| 148592 | pAc5.1B-EGFP-DmRoquin-del790-812_AE | Insect Expression of DmRoquin-del790-812 |
| 148593 | pAc5.1B-EGFP-DmRoquin-I793EL801E_AE | Insect Expression of DmRoquin-I793EL801E |
| 148594 | pAc5.1B-EGFP-DmRoquin-ILLVtoEEEE_AE | Insect Expression of DmRoquin-ILLVtoEEEE |
| 148595 | pAc5.1B-EGFP-DmRoquin-L801EL805E_AE | Insect Expression of DmRoquin-L801EL805E |
| 148596 | pAc5.1B-EGFP-DmRoquin-L805EV809E_AE | Insect Expression of DmRoquin-L805EV809E |
| 148598 | pAc5.1B-lambdaN-HA-DmNot1_1467-1717_AE | Insect Expression of DmNot1_1467-1717 |
| 148599 | pAc5.1B-lambdaN-HA-DmRoquin_501-819-del725-755_AE | Insect Expression of DmRoquin_501-819-del725-755 |
| 148600 | pAc5.1B-lambdaN-HA-DmRoquin_501-819-del790-812_AE | Insect Expression of DmRoquin_501-819-del790-812 |
| 148601 | pAc5.1B-lambdaN-HA-DmRoquin_501-819-ILLVtoEEEE_AE | Insect Expression of DmRoquin_501-819-ILLVtoEEEE |
| 148602 | pAc5.1B-lambdaN-HA-DmRoquin_702-819-ILLVtoEEEE_AE | Insect Expression of DmRoquin_702-819-ILLVtoEEEE |
| 148603 | pAc5.1B-lambdaN-HA-DmRoquin-ILLVtoEEEE_AE | Insect Expression of DmRoquin-ILLVtoEEEE |
| 148604 | pAc5.1B-lambdaN-HA-EGFP-DmRoquin_702-819_AE | Insect Expression of DmRoquin_702-819 |
| 148605 | pAc5.1B-lambdaN-HA-GST-DmRoquin_702-819_AE | Insect Expression of DmRoquin_702-819 |
| 148606 | pAc5.1B-lambdaN-HA-GST-DmRoquin_702-819-del725-755-del790-812_AE | Insect Expression of DmRoquin_702-819-del725-755-del790-812 |
| 148607 | pcDNA3.1-MS2-HA-DmRoquin-del725-755_AE | Mammalian Expression of DmRoquin-del725-755 |
| 148608 | pcDNA3.1-MS2-HA-DmRoquin-del725-755-del790-812_AE | Mammalian Expression of DmRoquin-del725-755-del790-812 |
| 148609 | pcDNA3.1-MS2-HA-DmRoquin-del790-812_AE | Mammalian Expression of DmRoquin-del790-812 |
| 148610 | pcDNA3.1-MS2-HA-DmRoquin-ILLVtoEEEE_AE | Mammalian Expression of DmRoquin-ILLVtoEEEE |
| 148611 | pcDNA3.1-MS2-HA-HsRoquin1_AE | Mammalian Expression of HsRoquin1 |
| 148612 | pcDNA3.1-MS2-HA-HsRoquin1_1-509_AE | Mammalian Expression of HsRoquin1_1-509 |
| 148613 | pcDNA3.1-MS2-HA-HsRoquin1_510-1133_AE | Mammalian Expression of HsRoquin1_510-1133 |
| 148614 | pcDNA3.1-MS2-HA-HsRoquin2_AE | Mammalian Expression of HsRoquin2 |
| 148615 | pcDNA3.1-MS2-HA-HsRoquin2_1-500_AE | Mammalian Expression of HsRoquin2_1-500 |
| 148616 | pcDNA3.1-MS2-HA-HsRoquin2_501-1191_AE | Mammalian Expression of HsRoquin2_501-1191 |
| 148617 | pnEA-NpH-HsNot9_19-285-V181E_AE | Bacterial Expression of HsNot9_19-285-V181E |
| 148618 | pnEA-NpH-HsNot9_19-285-Y134DG141W_AE | Bacterial Expression of HsNot9_19-285-Y134DG141W |
| 148619 | pnYC-NpM-DmCaf40_25-291_AE | Bacterial Expression of DmCaf40_25-291 |
| 148620 | pnYC-NpM-DmRoquin_501-702-GB1His6_AE | Bacterial Expression of DmRoquin_501-702 |
| 148621 | pnYC-NpM-DmRoquin_702-819-del725-755-del790-812-GB1His6_AE | Bacterial Expression of DmRoquin_702-819-del725-755-del790-812 |
| 148622 | pnYC-NpM-DmRoquin_702-819-del725-755-GB1His6_AE | Bacterial Expression of DmRoquin_702-819-del725-755 |
| 148623 | pnYC-NpM-DmRoquin_702-819-del790-812-GB1His6_AE | Bacterial Expression of DmRoquin_702-819-del790-812 |
| 148624 | pnYC-NpM-DmRoquin_702-819-GB1His6_AE | Bacterial Expression of DmRoquin_702-819 |
| 148625 | pnYC-NpM-DmRoquin_702-819-ILLVtoEEEE-GB1His6_AE | Bacterial Expression of DmRoquin_702-819-ILLVtoEEEE |
| 148626 | pnYC-NpM-DmRoquin_702-819-L805EV809E-GB1His6_AE | Bacterial Expression of DmRoquin_702-819-L805EV809E |
| 148627 | pnYC-NpM-DmRoquin_725-755_AE | Bacterial Expression of DmRoquin_725-755 |
| 148628 | pnYC-NpM-DmRoquin_790-810_AE | Bacterial Expression of DmRoquin_790-810 |
| 148629 | pnYC-NpM-HsNot1iso1_1093-2376_AE | Bacterial Expression of HsNot1iso1_1093-2376 |
| 148630 | pnYC-NpM-HsNot1iso1_1351-1588_AE | Bacterial Expression of HsNot1iso1_1351-1588 |
| 148631 | pnYC-NpM-HsNot1iso1_1607-1815_AE | Bacterial Expression of HsNot1iso1_1607-1815 |
| 148632 | pnYC-NpM-HsRoquin1_510-1133_AE | Bacterial Expression of HsRoquin1_510-1133 |
| 148633 | pT7-V5-SBP-C1-DmRoquin-del725-755_AE | Mammalian Expression of DmRoquin-del725-755 |
| 148634 | pT7-V5-SBP-C1-DmRoquin-del725-755-del790-812_AE | Mammalian Expression of DmRoquin-del725-755-del790-812 |
| 148635 | pT7-V5-SBP-C1-DmRoquin-del790-812_AE | Mammalian Expression of DmRoquin-del790-812 |
| 148636 | pT7-V5-SBP-C1-DmRoquin-ILLVtoEEEE_AE | Mammalian Expression of DmRoquin-ILLVtoEEEE |
| 148637 | pT7-V5-SBP-C1-HsRoquin1_AE | Mammalian Expression of HsRoquin1 |
| 148638 | pT7-V5-SBP-C1-HsRoquin1_501-1133_AE | Mammalian Expression of HsRoquin1_501-1133 |
| 148639 | pT7-V5-SBP-C1-HsRoquin2_AE | Mammalian Expression of HsRoquin2 |
| 148640 | pT7-V5-SBP-C1-HsRoquin2_1-500_AE | Mammalian Expression of HsRoquin2_1-500 |
| 148641 | pT7-V5-SBP-C1-HsRoquin2_501-1191_AE | Mammalian Expression of HsRoquin2_501-1191 |
| 148642 | pT7-V5-SBP-C1-HsRoquin_1-500_AE | Mammalian Expression of HsRoquin1_1-500 |
| 148699 | pnYC-NpH-HsNot1_1093-1317_AF | Bacterial Expression of HsNot1_1093-1317 |