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Structure of the PAN3 pseudokinase reveals the basis for interactions with the PAN2 deadenylase and the GW182 proteins.

Christie M, Boland A, Huntzinger E, Weichenrieder O, Izaurralde E
Mol Cell. 2013 Aug 8;51(3):360-73. doi: 10.1016/j.molcel.2013.07.011. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
147559pnYC-NpM-DmPAN3_349-655_TBacterial Expression of DmPAN3_349-655
147560pnYC-NpM-DmPAN3_349-790_TBacterial Expression of DmPAN3_349-790
147561pnYC-NpM-DmPAN3_349-790-C421F_TBacterial Expression of DmPAN3_349-790-C421F
147562pnYC-NpM-DmPAN3_349-790-C421FI555F_TBacterial Expression of DmPAN3_349-790-C421FI555F
147563pnYC-NpM-DmPAN3_349-790-F658EL662EL665E_TBacterial Expression of DmPAN3_349-790-F658EL662EL665E
147564pnYC-NpM-DmPAN3_349-790-I555F_TBacterial Expression of DmPAN3_349-790-I555F
147565pnYC-NpM-DmPAN3_349-790-L676EG683E_TBacterial Expression of DmPAN3_349-790-L676EG683E
147566pnYC-NpM-DmPAN3_349-790-M651F_TBacterial Expression of DmPAN3_349-790-M651F
147567pnYC-NpM-DmPAN3_349-790-M651FP652G_TBacterial Expression of DmPAN3_349-790-M651FP652G
147568pnYC-NpM-DmPAN3_349-790-M651H_TBacterial Expression of DmPAN3_349-790-M651H
147569pnYC-NpM-DmPAN3_349-790-M651HP652G_TBacterial Expression of DmPAN3_349-790-M651HP652G
147570pnYC-NpM-DmPAN3_349-790-P652G_TBacterial Expression of DmPAN3_349-790-P652G
147571pnYC-NpM-DmPAN3_641-790_TBacterial Expression of DmPAN3_641-790
147572pnYC-NpM-HsPAN3_245-687_TBacterial Expression of HsPAN3_245-687
147652pAc5.1B-EGFP-DmPAN3_349-655_UInsect Expression of DmPAN3_349-655
147653pAc5.1B-EGFP-DmPAN3_349-790_UInsect Expression of DmPAN3_349-790
147654pAc5.1B-EGFP-DmPAN3_656-790_UInsect Expression of DmPAN3_656-790
147655pAc5.1B-EGFP-DmPAN3-D575RL676EG683E_UInsect Expression of DmPAN3-D575RL676EG683E
147656pAc5.1B-EGFP-DmPAN3-F658EL662EL665E_UInsect Expression of DmPAN3-F658EL662EL665E
147657pAc5.1B-EGFP-DmPAN3-L676EG683E_UInsect Expression of DmPAN3-L676EG683E
147658pAc5.1B-EGFP-DmPAN3-L676EG683E-del460-465_UInsect Expression of DmPAN3-L676EG683E-del460-465
147659pAc5.1B-EGFP-DmPAN3-L676EG683EH739EK746ED748K_UInsect Expression of DmPAN3-L676EG683EH739EK746ED748K
147660pAc5.1B-EGFP-DmPAN3-L676EG683EK717SD721SH725S_UInsect Expression of DmPAN3-L676EG683EK717SD721SH725S
147661pAc5.1B-EGFP-DmPAN3-L676EG683ER760TD761T_UInsect Expression of DmPAN3-L676EG683ER760TD761T
147662pAc5.1B-EGFP-DmPAN3-L676EG683ES708AT710A_UInsect Expression of DmPAN3-L676EG683ES708AT710A
147663pAc5.1B-EGFP-DmPAN3-L676EG683ET408FC421FI555F_UInsect Expression of DmPAN3-L676EG683ET408FC421FI555F
147664pAc5.1B-EGFP-DmPAN3-M651FP652G_UInsect Expression of DmPAN3-M651FP652G
147665pAc5.1B-EGFP-DmPAN3-R429DL676EG683E_UInsect Expression of DmPAN3-R429DL676EG683E
147666pAc5.1B-EGFP-DmPAN3-T408FC421FI555F_UInsect Expression of DmPAN3-T408FC421FI555F
147667pAc5.1B-lambdaN-HA-DmPAN3-5xmut_UInsect Expression of DmPAN3-5xmut
147668pAc5.1B-lambdaN-HA-DmPAN3-7xmut_UInsect Expression of DmPAN3-7mut
147669pAc5.1B-lambdaN-HA-DmPAN3-10xmut_UInsect Expression of DmPAN3-10mut
147670pAc5.1B-lambdaN-HA-DmPAN3-D575RL676EG683E_UInsect Expression of DmPAN3-D575RL676EG683E
147671pAc5.1B-lambdaN-HA-DmPAN3-F658EL662EL665E_UInsect Expression of DmPAN3-F658EL662EL665E
147672pAc5.1B-lambdaN-HA-DmPAN3-L676EG683E_UInsect Expression of DmPAN3-L676EG683E
147673pAc5.1B-lambdaN-HA-DmPAN3-L676EG683E-del460-465_UInsect Expression of DmPAN3-L676EG683E-del460-465
147674pAc5.1B-lambdaN-HA-DmPAN3-L676EG683EH739EK746ED748K_UInsect Expression of DmPAN3-L676EG683EH739EK746ED748K
147675pAc5.1B-lambdaN-HA-DmPAN3-M651FP652G_UInsect Expression of DmPAN3-M651FP652G
147676pAc5.1B-lambdaN-HA-DmPAN3-R429DL676EG683E_UInsect Expression of DmPAN3-R429DL676EG683E
147677pAc5.1B-lambdaN-HA-DmPAN3-T408FC421FI555F_UInsect Expression of DmPAN3-T408FC421FI555F
147678pEGFP-C1-HsTNRC6C_1208-1690-CIM1CIM2mut_UMammalian Expression of HsTNRC6C_1208-1690-CIM1CIM2mut
147679pEGFP-C1-HsTNRC6C_1208-1690-M2WA_UMammalian Expression of HsTNRC6C_1208-1690-M2WA
147680pnEA-NpM-NcPAN3_234-656_UBacterial Expression of NcPAN3_234-656
147681pnEA-NpM-NcPAN3_234-656-GW-mut_UBacterial Expression of NcPAN3_234-656-GW-mut
147682pnYC-NpM-HsPAN3_245-687-F553EL557EA560E_UBacterial Expression of HsPAN3_245-687-F553EL557EA560E
147683pnYC-NpM-HsPAN3_245-687-L571EG578E_UBacterial Expression of HsPAN3_245-687-L571EG578E
147684pnYC-NpM-HsPAN3_245-687-M546F_UBacterial Expression of HsPAN3_245-687-M546F
147685pnYC-NpM-HsPAN3_245-687-M546FP547G_UBacterial Expression of HsPAN3_245-687-M546FP547G
147686pnYC-NpM-HsPAN3_245-687-M546H_UBacterial Expression of HsPAN3_245-687-M546H
147687pT7-EGFP-C1-HsPAN3-6xmut_UMammalian Expression of HsPAN3-6xmut
147688pT7-EGFP-C1-HsPAN3-C319FL447F_UMammalian Expression of HsPAN3-C319FL447F
147689pT7-EGFP-C1-HsPAN3-F553EL557EA560E_UMammalian Expression of HsPAN3-F553EL557EA560E
147690pT7-EGFP-C1-HsPAN3-F553EL557EA560ER655TD656T_UMammalian Expression of HsPAN3-F553EL557EA560ER655TD656T
147691pT7-EGFP-C1-HsPAN3-F553EL557EA560ES603AT605A_UMammalian Expression of HsPAN3-F553EL557EA560E
147692pT7-EGFP-C1-HsPAN3-L571EG578E_UMammalian Expression of HsPAN3-L571EG578E
147693pT7-EGFP-C1-HsPAN3-M546FP547G_UMammalian Expression of HsPAN3-M546FP547G
147694pT7-V5-SBP-C1-HsPAN3_245-550_UMammalian Expression of HsPAN3_245-550
147695pT7-V5-SBP-C1-HsPAN3_245-687_UMammalian Expression of HsPAN3_245-687
147696pT7-V5-SBP-C1-HsPAN3_551-687_UMammalian Expression of HsPAN3_551-687

Antibodies from Article