Skip to main content
Addgene

A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing.

Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, Weichenrieder O, Izaurralde E
Mol Cell. 2014 Jun 5;54(5):737-50. doi: 10.1016/j.molcel.2014.03.034. Epub 2014 Apr 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
146701pAc5.1B-EGFP-DmNot1_1710-2505_JInsect Expression of DmNot1_1710-2505
146702pAc5.1B-lambdaN-HA-DmNot1_1710-2505_JInsect Expression of DmNot1_1710-2505
147098pCIneo-lambdaN-HA-HsRCK_1-295_OMammalian Expression of HsRck_1-295
147099pCIneo-lambdaN-HA-HsRCK_296-472_OMammalian Expression of HsRck_296-472
147480pLNHA-C1-HsNot9_18-285_SMammalian Expression of HsNot9_18-285
147481pnEA-NpH-HsNot9_19-285_SBacterial Expression of HsNot9_19-285
147482pT7-EGFP-C1-HsNot9_18-285_SMammalian Expression of HsNot9_18-285
147573pnEA-NpH-HsNot1iso1_1093-1317_TBacterial Expression of HsNot1iso1_1093-1317
147574pnEA-NpM-HsNot1iso1_1093-1317-K1208AH1212AK1218A_TBacterial Expression of HsNot1iso1_1093-1317-K1208AH1212AK1218A
147773pnEA-NpH-HsNot9_19-285-P165AY203AR205AR244A_VBacterial Expression of HsNot9_19-285-P165AY203AR244A
147774pnEA-NpH-HsNot9_19-285-R205AH208A_VBacterial Expression of HsNot9_19-285-R205AH208A
147775pnEA-NpH-HsNot9_19-285-Y203AR205AH208AA248F_VBacterial Expression of HsNot9_19-285-Y203AR244AH208AA248F
147776pnEA-NpH-HsNot9_19-285-Y203AR205AR244AH208A_VBacterial Expression of HsNot9_19-285-Y203AR244AR244AH208A
147777pnEA-NpH-HsNot9_19-285-Y203AR244A_VBacterial Expression of HsNot9_19-285-Y203AR244A
147778pnYC-NpM-HsNot1iso1_1356-1561_VBacterial Expression of HsNot1iso1_1356-1561
147779pnYC-NpM-HsNot1iso1_1356-1581_VBacterial Expression of HsNot1iso1_1356-1581
147780pnYC-NpM-HsNot1iso1_1356-1607_VBacterial Expression of HsNot1iso1_1356-1607
147781pnYC-NpM-HsNot1iso1_1356-1628_VBacterial Expression of HsNot1iso1_1356-1628
147829pAc5.1B-lambdaN-HA-DmCaf40-NFCVYAtoAAYYAF_WInsect Expression of DmCaf40-NFCVYAtoAAYYAF
147830pAc5.1B-lambdaN-HA-DmGW182_1-634_WInsect Expression of DmGW182_1-634
147831pAc5.1B-lambdaN-HA-DmGW182_635-1384_WInsect Expression of DmGW182_635-1384
147832pMT-CTAP-PrP-SBP-DmCaf40_WInsect Expression of DmCaf40
147833pMT-CTAP-PrP-SBP-DmCaf40-N63AF65AC69YV76Y_WInsect Expression of DmCaf40-N63AF65AC69YV76Y
147834pMT-CTAP-PrP-SBP-DmCaf40-NFCVYAtoAAYYAF_WInsect Expression of DmCaf40-NFCVYAtoAAYYAF
147835pMT-EGFP-CTAP-PrP-SBP_WInsect Expression of EGFP
147836pT7-EGFP-C1-HsNot1iso1-del1097-1110_WMammalian Expression of HsNot1iso1-del1097-1110
147837pT7-EGFP-C1-HsNot1iso1-IKGIRQtoDSYDAA-shRNAres_WMammalian Expression of HsNot1iso1-IKGIRQtoDSYDAA-shRNAres
147838pAc5.1B-EGFP-DmCaf40-NFCVYAtoAAYYAF_WInsect Expression of DmCaf40-NFCVYAtoAAYYAF
147839pAc5.1B-EGFP-DmCaf40-R210AH213A_WInsect Expression of DmCaf40-R210AH213A
147840pAc5.1B-EGFP-DmMe31B-Q294AN296F-dsRNAres_WInsect Expression of DmMe31B-Q294AAN296F-dsRNAres
147841pAc5.1B-EGFP-DmMe31B-Q294AN296FR347A-dsRNAres_WInsect Expression of DmMe31B-Q294AAN296FR347A-dsRNAres
147842pAc5.1B-EGFP-DmMe31B-R347A-dsRNAres_WInsect Expression of DmMe31B-R347A-dsRNAres
147843pAc5.1B-EGFP-DmMe31B-S291A-dsRNAres_WInsect Expression of DmMe31B-S291A-dsRNAres
147844pAc5.1B-EGFP-DmNot1_1-812_WInsect Expression of DmNot1_1-812
147845pAc5.1B-EGFP-DmNot1_812-1945-dsRNAres_WInsect Expression of DmNot1_812-1945-dsRNAres
147846pAc5.1B-EGFP-DmNot1_812-1945-K1537SG1562EQ1658A-dsRNAres_WInsect Expression of DmNot1_812-1945-K1537SG1562EQ1658A-dsRNAres
147847pAc5.1B-EGFP-DmNot1_1387-1699_WInsect Expression of DmNot1_1387-1699
147848pAc5.1B-EGFP-DmNot1_1387-1717_WInsect Expression of DmNot1_1387-1717
147849pAc5.1B-EGFP-DmNot1_1467-1699_WInsect Expression of DmNot1_1467-1699
147850pAc5.1B-EGFP-DmNot1_1467-1717_WInsect Expression of DmNot1_1467-1717
147851pAc5.1B-EGFP-DmNot1_1467-1717-G1562E_WInsect Expression of DmNot1_1467-1717-G1562E
147852pAc5.1B-EGFP-DmNot1_1467-1717-G1562Y_WInsect Expression of DmNot1_1467-1717-G1562Y
147853pAc5.1B-EGFP-DmNot1_1467-1717-K1537S_WInsect Expression of DmNot1_1467-1717-K1537S
147854pAc5.1B-EGFP-DmNot1_1467-1717-K1537SG1562EQ1658A_WInsect Expression of DmNot1_1467-1717-K1537SG1562EQ1658A
147855pAc5.1B-EGFP-DmNot1_1467-1717-K1569A_WInsect Expression of DmNot1_1467-1717-K1569A
147856pAc5.1B-EGFP-DmNot1_1467-1717-Q1658A_WInsect Expression of DmNot1_1467-1717-Q1658A
147857pAc5.1B-EGFP-DmNot1_1497-1644_WInsect Expression of DmNot1_1497-1644
147858pAc5.1B-EGFP-DmNot1_1941-2505-dsRNAres_WInsect Expression of DmNot1_1941-2505-dsRNAres
147859pAc5.1B-EGFP-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_WInsect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAres
147860pAc5.1B-EGFP-DmNot1-K1537SG1562EQ1658A-dsRNAres_WInsect Expression of DmNot1-K1537SG1562EQ1658A-dsRNAres
147861pAc5.1B-EGFP-DmNot1-RNFtoAAA-dsRNAres_WInsect Expression of DmNot1-RNFtoAAA-dsRNAres
147862pAc5.1B-HA-DmMe31B-Q294AN296F-dsRNAres_WInsect Expression of DmMe31B-Q294AAN296F-dsRNAres
147863pAc5.1B-HA-DmMe31B-Q294AN296FR347A-dsRNAres_WInsect Expression of DmMe31B-Q294AAN296FR347A-dsRNAres
147864pAc5.1B-HA-DmMe31B-R347A-dsRNAres_WInsect Expression of DmMe31B-R347A-dsRNAres
147865pAc5.1B-HA-DmMe31B-S291A-dsRNAres_WInsect Expression of DmMe31B-S291A-dsRNAres
147866pAc5.1B-lambdaN-HA-DmCaf40_25-291_WInsect Expression of DmCaf40_25-291
147867pAc5.1B-lambdaN-HA-DmNot1_1467-1717-K1537S_WInsect Expression of DmNot1_1467-1717-K1537S
147868pAc5.1B-lambdaN-HA-DmNot1_1467-1717-K1537SG1562EQ1658A_WInsect Expression of DmNot1_1467-1717-K1537SG1562EQ1658A
147869pAc5.1B-lambdaN-HA-DmNot1_1467-1717-K1537SQ1658A_WInsect Expression of DmNot1_1467-1717-K1537SQ1658A
147870pAc5.1B-lambdaN-HA-DmNot1_1467-1717-K1569A_WInsect Expression of DmNot1_1467-1717-K1569A
147871pAc5.1B-lambdaN-HA-DmNot1_1467-1717-Q1658A_WInsect Expression of DmNot1_1467-1717-Q1658A
147872pAc5.1B-lambdaN-HA-DmNot1_1497-1644_WInsect Expression of DmNot1_1497-1644
147873pAc5.1B-lambdaN-HA-DmNot1_1497-1717-G1562E_WInsect Expression of DmNot1_1497-1717-G1562E
147874pAc5.1B-lambdaN-HA-DmNot1_1497-1717-G1562Y_WInsect Expression of DmNot1_1497-1717-G1562Y
147875pAc5.1B-lambdaN-HA-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_WInsect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAres
147876pAc5.1B-lambdaN-HA-DmNot1-K1537SG1562EQ1658A-dsRNAres_WInsect Expression of DmNot1-K1537SG1562EQ1658A-dsRNAres
147877pAc5.1B-lambdaN-HA-DmNot1-K1537SQ1658A-dsRNAres_WInsect Expression of DmNot1-K1537SQ1658A-dsRNAres
147878pAc5.1B-lambdaN-HA-DmNot1-RNFtoAAA-dsRNAres_WInsect Expression of DmNot1-RNFtoAAA-dsRNAres
147879pCIneo-lambdaN-HsNot1_1318-1605-HA-MBP_WMammalian Expression of HsNot1iso1_1318-1605
147881pLNHA-C1-HsNot1iso1-del1097-1110-R1138A-shRNAres_WMammalian Expression of HsNot1iso1-del1097-1110-R1138A-shRNAres
147882pLNHA-C1-HsNot1iso1-del1097-1110-shRNAres_WMammalian Expression of HsNot1iso1-del1097-1110-shRNAres
147883pnYC-NpM-HsNot1iso1_1833-2361-F2353D_WBacterial Expression of HsNot1iso1_1833-2361-F2353D
147884pnYC-NpM-HsNot1iso1_1833-2361-I2330E_WBacterial Expression of HsNot1iso1_1833-2361-I2330E
147885pSUPERpuro-BglII-HsNot9shRNA2_WMammalian Expression of shRNA2-HsNot9
147886pT7-EGFP-C1-HsNot1iso1_1-1605-del1079-1110-RNFtoAAA-shRNAres_WMammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFtoAAA-shRNAres
147887pT7-EGFP-C1-HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres_WMammalian Expression of HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres
147888pT7-EGFP-C1-HsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAres_WMammalian Expression of HsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAres
147889pT7-EGFP-C1-HsNot1iso1_1-1605-shRNAres_WMammalian Expression of HsNot1iso1_1-1605-shRNAres
147890pT7-EGFP-C1-HsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAres_WMammalian Expression of HsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAres
147891pT7-EGFP-C1-HsNot1iso1_1-1840-shRNAres_WMammalian Expression of HsNot1iso1_1-1840-shRNAres
147892pT7-EGFP-C1-HsNot1iso1_1093-1317_WMammalian Expression of HsNot1iso1_1093-1317 *Please note that this plasmid encodes amino acids 1093-1322 (not 1093-1317 as the name suggests).
147893pT7-EGFP-C1-HsNot1iso1_1318-1605_WMammalian Expression of HsNot1iso1_1318-1605
147894pT7-EGFP-C1-HsNot1iso1_1318-1605-IKGIRQtoDSYDAA_WMammalian Expression of HsNot1iso1_1318-1605-IKGIRQtoDSYDAA
147895pT7-EGFP-C1-HsNot1iso1_1318-1605-IKGRQtoDSYAA_WMammalian Expression of HsNot1iso1_1318-1605-IKGRQtoDSYAA
147896pT7-EGFP-C1-HsNot1iso1_1318-1605-KGRQtoSYAA_WMammalian Expression of HsNot1iso1_1318-1605-KGRQ-SYAA
147897pT7-EGFP-C1-HsNot1iso1-del1097-1110-IKGRQtoDSYAA-shRNAres_WMammalian Expression of HsNot1iso1-del1097-1110-IKGRQtoDSYAA-shRNAres
147898pT7-EGFP-C1-HsNot1iso1-del1097-1110-NF1144AA-shRNAres_WMammalian Expression of HsNot1iso1-del1097-1110-NF1144AA-shRNAres
147899pT7-EGFP-C1-HsNot1iso1-del1097-1110-R1138A-shRNAres_WMammalian Expression of HsNot1iso1-del1097-1110-R1138A-shRNAres
147900pT7-EGFP-C1-HsNot1iso1-IKGRQtoDSYAA-shRNAres_WMammalian Expression of HsNot1iso1-IKGRQtoDSYAA-shRNAres
147901pT7-EGFP-C1-HsNot1iso1-KGRQtoSYAA-shRNAres_WMammalian Expression of HsNot1iso1-KGRQtoSYAA-shRNAres
147902pT7-EGFP-C1-HsNot9-H58AF60AA64YV71Y-shRNA1res_WMammalian Expression of HsNot9-H58AF60AA64YV71Y-shRNA1res
147903pT7-EGFP-C1-HsNot9-R205AH208A-shRNA1res_WMammalian Expression of HsNot9-R205AH208A-shRNA1res
147904pT7-EGFP-C1-HsNot9-shRNAres1_WMammalian Expression of HsNot9-shRNAres1
147905pT7-EGFP-C1-HsNot9-shRNAres2_WMammalian Expression of HsNot9-shRNAres2
147906pT7-EGFP-C1-HsNot9-shRNAres2-H58AF60AA64YV71Y_WMammalian Expression of HsNot9-shRNAres2-H58AF60AA64YV71Y
147907pT7-EGFP-C1-HsNot9-Y203AR205AH208AR244A-shRNA1res_WMammalian Expression of HsNot9-Y203AR205AH208AR244A-shRNA1res
147908pT7-EGFP-C1-HsNot9-Y203AR244A-shRNA1res_WMammalian Expression of HsNot9-Y203AR244A-shRNA1res
147973pAc5.1B-EGFP-DmCaf40-A253FY208A_XInsect Expression of DmCaf40-A253FY208A
147974pAc5.1B-EGFP-DmCaf40-F65AC69Y_XInsect Expression of DmCaf40-F65AC69Y
147975pAc5.1B-EGFP-DmCaf40-N63AF65AC69Y_XInsect Expression of DmCaf40-N63AF65AC69Y
147976pAc5.1B-EGFP-DmCaf40-N63AF65AC69YV76Y_XInsect Expression of DmCaf40-N63AF65AC69YV76Y
147977pAc5.1B-EGFP-DmCaf40-N63AF65AC69YV76YR210AH213A_XInsect Expression of DmCaf40-N63AF65AC69YV76YR210AH213A
147978pAc5.1B-EGFP-DmCaf40-NFCVYRHAtoAAYYAAAF_XInsect Expression of DmCaf40-NFCVYRHAtoAAYAAAF
147979pAc5.1B-EGFP-DmCaf40-R249H_XInsect Expression of DmCaf40-R249H
147980pAc5.1B-EGFP-DmCaf40-Y208AR210AH213AA253F_XInsect Expression of DmCaf40-Y208AR210AH213AA253F
147981pAc5.1B-EGFP-DmCaf40-Y208AR249A_XInsect Expression of DmCaf40-Y208AR249A
147982pAc5.1B-EGFP-DmNot1_1-1717_XInsect Expression of DmNot1_1-1717
147983pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169_XInsect Expression of DmNot1_1-1717-del1156-1169
147984pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169-R1197AN1203AF1204A_XInsect Expression of DmNot1_1-1717-del1156-1169-R1197AN1203AF1204A
147985pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA_XInsect Expression of DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA
147986pAc5.1B-EGFP-DmNot1_1-1717-K1537SG1562EQ1658A_XInsect Expression of DmNot1_1-1717-K1537SG1562EQ1658A
147987pAc5.1B-EGFP-DmNot1_1-1717-R1197AN1203AF1204A_XInsect Expression of DmNot1_1-1717-R1197AN1203AF1204A
147988pAc5.1B-EGFP-DmNot1_1-1717-RNFKGQtoAAASEA_XInsect Expression of DmNot1_1-1717-RNFKGQtoAAASEA
147989pAc5.1B-EGFP-DmNot1_1-1945-dsRNAres_XInsect Expression of DmNot1_1-1945-dsRNAres
147990pAc5.1B-EGFP-DmNot1_1-1945-K1537SG1562EQ1658A-dsRNAres_XInsect Expression of DmNot1_1-1945-K1537SG1562EQ1658A-dsRNAres
147991pAc5.1B-EGFP-DmNot1-del1156-1169-dsRNAres_XInsect Expression of DmNot1-del1156-1169-dsRNAres
147992pAc5.1B-lambdaN-HA-DmCaf40-A253FY208A_XInsect Expression of DmCaf40-A253FY208A
147993pAc5.1B-lambdaN-HA-DmCaf40-F65AC69Y_XInsect Expression of DmCaf40-F65AC69Y
147994pAc5.1B-lambdaN-HA-DmCaf40-R249H_XInsect Expression of DmCaf40-R249H
147995pAc5.1B-lambdaN-HA-DmCaf40-Y208AR249A_XInsect Expression of DmCaf40-Y208AR249A
147996pAc5.1B-lambdaN-HA-DmNot1-G1562E-dsRNAres_XInsect Expression of DmNot1-G1562E-dsRNAres
147997pLNHA-C1-HsNot1iso1_840-1605_XMammalian Expression of HsNot1iso1_840-1605
147998pLNHA-C1-HsNot1iso1_840-1605-del1097-1110_XMammalian Expression of HsNot1iso1_840-1605-del1097-1110
147999pLNHA-C1-HsNot1iso1_840-1605-del1097-1110-IKGRQtoDSYAA_XMammalian Expression of HsNot1iso1_840-1605-del1097-1110-IKGRQtoDSYAA
148000pLNHA-C1-HsNot1iso1_840-1605-del1097-1110-RNFtoAAA_XMammalian Expression of HsNot1iso1_840-1605-del1097-1110-RNFtoAAA
148001pLNHA-C1-HsNot1iso1_840-1605-IKGRQtoDSYAA_XMammalian Expression of HsNot1iso1_840-1605-IKGRQtoDSYAA
148475pAc5.1B-lambdaN-HA-DmNot1_1149-1376_ACInsect Expression of DmNot1_1149-1376
148503pCIneo-lambdaN-HsNot1iso1_1318-1605-HA-MBP_ADMammalian Expression of HsNot1iso1_1318-1605

Antibodies from Article