miRISC and the CCR4-NOT complex silence mRNA targets independently of 43S ribosomal scanning.
Kuzuoglu-Ozturk D, Bhandari D, Huntzinger E, Fauser M, Helms S, Izaurralde E
EMBO J. 2016 Jun 1;35(11):1186-203. doi: 10.15252/embj.201592901. Epub 2016 Mar 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 146456 | pT7-EGFP-C1-HsRCK-E236Q_H | Mammalian Expression of HsRck-E236Q |
| 147338 | pAc5.1B-HA-DmDCP1-GSSGmut-dsRNAres_Q | Insect Expression of DmDCP1-GSSGmut-dsRNAres |
| 148386 | pAc5.1B-EGFP-DmMe31B-QHTKRKKtoAAAAAAA_AC | Insect Expression of DmMe31B-QHTRKKtoAAAAAA |
| 148387 | pAc5.1B-EGFP-DmMe31B-R307AK313AK314A_AC | Insect Expression of DmMe31B-R307AK313AK314A |
| 148388 | pAc5.1B-HA-DmMe31B-E208A-dsRNAres_AC | Insect Expression of DmMe31B-E208A-dsRNAres |
| 148389 | pAc5.1B-HA-DmMe31B-K108A-dsRNAres_AC | Insect Expression of DmMe31B-K108A-dsRNAres |
| 148390 | pAc5.1B-HA-DmMe31B-QHTKRKKtoAAAAAAA-dsRNAres_AC | Insect Expression of DmMe31B-QHTKRKKtoAAAAAAA-dsRNAres |
| 148391 | pAc5.1B-HA-DmMe31B-QHTKtoAAAA-dsRNAres_AC | Insect Expression of DmMe31B-QHTKtoAAAA-dsRNAres |
| 148392 | pAc5.1B-HA-DmMe31B-R307AK313AK314A-dsRNAres_AC | Insect Expression of DmMe31B-R307AK313AK314A |
| 148393 | pAc5.1B-HA-DmMe31B-RFQNRtoEDAFA-dsRNAres_AC | Insect Expression of DmMe31B-RFQNRtoEDAFA-dsRNAres |
| 148394 | pAc5.1B-lambdaN-HA-DmAGO1-L819Y-siRNAres_AC | Insect Expression of DmAGO1-L819Y-siRNAres |
| 148395 | pAc5.1B-lambdaN-HA-DmAGO1-P714GA744FL819Y-siRNAres_AC | Insect Expression of DmAGO1-P714GA744FL819Y-siRNAres |
| 148396 | pAc5.1B-lambdaN-HA-DmMe31B-QHTKRKKtoAAAAAAA_AC | Insect Expression of DmMe31B-QHTKRKKtoAAAAAAA |
| 148397 | pAc5.1B-lambdaN-HA-DmMe31B-R307AK313AK314A_AC | Insect Expression of DmMe31B-R307AK313AK314A |
| 148398 | pAc5.1B-lambdaN-HA-DmNot1_1147-1673_AC | Insect Expression of DmNot1_1147-1673 |
| 148399 | pAc5.1B-lambdaN-HA-DmNot1_1149-1962-dsRNAres_AC | Insect Expression of DmNot1_1149-1962-dsRNAres |
| 148400 | pAc5.1B-lambdaN-HA-DmNot1_1377-1673_AC | Insect Expression of DmNot1_1377-1673 |
| 148401 | pAc5.1B-lambdaN-HA-DmNot1_1377-1962-dsRNAres_AC | Insect Expression of DmNot1_1377-1962-dsRNAres |
| 148402 | pAc5.1B-lambdaN-HA-DmNot1_1674-1962-dsRNAres_AC | Insect Expression of DmNot1_1674-1962-dsRNAres |
| 148403 | pAc5.1B-lambdaN-HA-DmNot1_1963-2505-dsRNAres_AC | Insect Expression of Dmot1_1963-2502-dsRNAres |
| 148404 | pAc5.1B-lambdaN-HA-DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNAres_AC | Insect Expression of DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNares |
| 148405 | pAc5.1B-no5UTR-lambdaN-HA-GST-V5His6_AC | Insect Expression vector |
| 148406 | pAc5.1C-Fluc-MtoL-V5-5BoxB-A95-HhR_AC | Insect Expression of 5BoxB-A95-HhR |
| 148407 | pAc5.1C-Fluc-Nerfin-PolyA-HhR_AC | Insect Expression of Nerfin-PolyA-HhR |
| 148408 | pAc5.1C-no5UTR-Fluc-5BoxB_AC | Insect Expression of 5BoxB |
| 148409 | pAc5.1C-no5UTR-Fluc-MtoL-V5-5BoxB-polyA-HhR_AC | Insect Expression of 5BoxB-polyA-HhR |
| 148410 | pcDNA3.1-MS2-HA-HA-HsRCK-RFQNRtoEDAAA_AC | Mammalian Expression of HsRck-RFQNRtoEDAAA |
| 148411 | pcDNA3.1-MS2-HA-HsNot1iso1_1-1084-shRNAres_AC | Mammalian Expression of HsNot1iso1_1-1084-shRNAres |
| 148412 | pcDNA3.1-MS2-HA-HsNot1iso1_1085-1317-shRNAres_AC | Mammalian Expression of HsNot1iso1_1085-1317-shRNAres |
| 148413 | pcDNA3.1-MS2-HA-HsNot1iso1_1085-1605_AC | Mammalian Expression of HsNot1iso1_1085-1605 |
| 148414 | pcDNA3.1-MS2-HA-HsNot1iso1_1318-1605-shRNAres_AC | Mammalian Expression of HsNot1iso1_1318-1605-shRNAres |
| 148415 | pcDNA3.1-MS2-HA-HsNot1iso1_1606-1840-shRNAres_AC | Mammalian Expression of HsNot1iso1_1606-1840-shRNAres |
| 148416 | pcDNA3.1-MS2-HA-HsNot1iso1_1841-2376-shRNAres_AC | Mammalian Expression of HsNot1iso1_1841-2376-shRNAres |
| 148417 | pcDNA3.1-MS2-HA-HsRck_AC | Mammalian Expression of HsRck |
| 148418 | pcDNA3.1-MS2-HA-HsRck_1-295_AC | Mammalian Expression of HsRck_1-295 |
| 148419 | pcDNA3.1-MS2-HA-HsRck_296-472_AC | Mammalian Expression of HsRck_296-472 |
| 148420 | pcDNA3.1-MS2-HA-HsRck_296-472-10xmut_AC | Mammalian Expression of HsRck_296-472-10xmut |
| 148421 | pcDNA3.1-MS2-HA-HsRck_296-472-QHTRtoAAAA_AC | Mammalian Expression of HsRck_296-472-QHTRtoAAAA |
| 148422 | pcDNA3.1-MS2-HA-HsRck_296-472-RKKtoAAA_AC | Mammalian Expression of HsRck_296-472-RKKtoAAA |
| 148423 | pcDNA3.1-MS2-HA-HsRck_296-472-RQNtoAAA_AC | Mammalian Expression of HsRck_296-472-RQNtoAAA |
| 148424 | pcDNA3.1-TISU-HA-hRLuc-V5-6xbsMS2_AC | Mammalian Expression of TISU-HA-hRluc-V5-6xbsMS2 |
| 148425 | pCIneo-CAA-RLuc-6xbsMS2-A95-Malat_AC | Mammalian Expression of 6xbsMS2-A95-Malat |
| 148426 | pCIneo-GQ-RLuc-6xbsMS2-A95-Malat_AC | Mammalian Expression of 6xbsMS2-A95-Malat |
| 148427 | pCIneo-lambdaN-HA-HsAGO2-K663AL697Y_AC | Mammalian Expression of HsAGO2-K663AL697Y |
| 148428 | pCIneo-lambdaN-HA-HsAGO2-P593GA623FK663AL697Y_AC | Mammalian Expression of HsAGO2-P593GA623FK663AL697Y |
| 148429 | pCIneo-lambdaN-HA-HsRCK-RFQNRtoEDAAA_AC | Mammalian Expression of HsRck-RFQNRtoEDAAA |
| 148430 | pCIneo-Rluc-6xbsMS2-polyA-Malat_AC | Mammalian Expression of 6xbsMS2-polyA-Malat |
| 148431 | pCIneo-Rluc-6xbsMS2-random_AC | Mammalian Expression of 6xbsMS2-random |
| 148432 | pCIneo-RLuc-5BoxB-polyA-Malat_AC | Mammalian Expression of 5BoxB-polyA-Malat |
| 148433 | pCIneo-RLuc-6xbsMS2-8xlet7_AC | Mammalian Expression of 6xbsMS2-8xlet7 |
| 148434 | pCIneo-TISU-RLuc-6xbsMS2_AC | Mammalian Expression of 6xbsMS2 |
| 148435 | pCIneo-TISU-RLuc-6xbsMS2-8xlet7_AC | Mammalian Expression of 6xbsMS2-8xlet7 |
| 148436 | pCIneo-TISU-RLuc-6xbsMS2-polyA-Malat_AC | Mammalian Expression of 6xbsMS2-polyA-Malat |
| 148437 | pCIneo-TISU-RLuc-6xbsMS2-random_AC | Mammalian Expression of 6xbsMS2-random |
| 148438 | pCIneo-V5-SBP-HseIF4AI_AC | Mammalian Expression of HseIF4AI |
| 148439 | pCIneo-V5-SBP-HseIF4AII_AC | Mammalian Expression of HseIF4AII |
| 148440 | pCMV-TAPtagN-PrP-SBP-HsAGO2_AC | Mammalian Expression of HsAGO2 |
| 148441 | pLNHA-C1-HseIF1_AC | Mammalian Expression of HseIF1 |
| 148442 | pLNHA-C1-HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres_AC | Mammalian Expression of HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres |
| 148443 | pRL-TK-hFluc-CrPV-hRluc-6xbsMS2-Malat_AC | Mammalian Expression of 6xbsMS2-Malat |
| 148444 | pRL-TK-hFluc-HCV-hRluc-6xbsMS2-Malat_AC | Mammalian Expression of 6xbsMS2-Malat |
| 148445 | pSUPERpuro-BglII-Hs4E-T_AC | Mammalian Expression of Hs4E-T-shRNA |
| 148446 | pT7-EGFP-C1-CrPV-Rluc-6xbsMS2-PolyA-Malat_AC | Mammalian Expression of 6xbsMS2-PolyA-Malat |
| 148447 | pT7-EGFP-C1-HCV-Fluc_AC | Mammalian Expression of Fluc |
| 148448 | pT7-EGFP-C1-HCV-RLuc-6xbsMS2-polyA-Malat_AC | Mammalian Expression of 6xbsMS2-polyA-Malat |
| 148449 | pT7-EGFP-C1-HseIF1_AC | Mammalian Expression of HseIF1 |
| 148450 | pT7-EGFP-C1-HsRCK-E236Q-shRNAres_AC | Mammalian Expression of HSRck-E236Q-shRNAres |
| 148451 | pT7-EGFP-C1-HsRCK-K135A-shRNAres_AC | Mammalian Expression of HsRck-K135A-shRNAres |
| 148452 | pT7-EGFP-C1-HsRCK-QHTRRKKtoAAAAAAA-shRNAres_AC | Mammalian Expression of HsRck-QHTRRKKtoAAAAAAA-shRNAres |
| 148453 | pT7-EGFP-C1-HsRCK-QHTRtoAAAA-shRNAres_AC | Mammalian Expression of HsRck-QHTRtoAAAA-shRNAres |
| 148454 | pT7-EGFP-C1-HsRCK-RFQNRtoEDAAA-shRNAres_AC | Mammalian Expression of HsRck-RFQNRtoEDAAA-shRNAres |
| 148499 | pAc5.1C-no5UTR-Fluc-5BoxB-PolyA-HhR_AD | Insect Expression of 5BoxB-PolyA-HhR |