An unusual arrangement of two 14-3-3-like domains in the SMG5-SMG7 heterodimer is required for efficient nonsense-mediated mRNA decay.
Jonas S, Weichenrieder O, Izaurralde E
Genes Dev. 2013 Jan 15;27(2):211-25. doi: 10.1101/gad.206672.112.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 146191 | pCIneo-RLuc-BGG-PTC_E | Mammalian Expression of BGG-PTC |
| 146192 | pCIneo-RLuc-BGG-WT_E | Mammalian Expression of BGG-WT |
| 147456 | pCIneo-lambdaN-HA-CeSmg5_S | Mammalian Expression of CeSmg5 |
| 147457 | pCIneo-lambdaN-HA-CeSmg5-G91E_S | Mammalian Expression of CeSmg5-G91E |
| 147458 | pCIneo-lambdaN-HA-CeSmg5-H116E_S | Mammalian Expression of CeSmg5-H116E |
| 147459 | pCIneo-lambdaN-HA-CeSmg5-Q94E_S | Mammalian Expression of CeSmg5-Q94E |
| 147460 | pCIneo-lambdaN-HA-HsSmg6-sh(t10)RNAres_S | Mammalian Expression of HsSmg6-sh(10)RNAres |
| 147461 | pCIneo-lambdaN-HA-HsSmg7-G100E-siRNAres-shRNAres_S | Mammalian Expression of HsSmg7-G100E-siRNAres-shRNAres |
| 147462 | pCIneo-lambdaN-HA-HsSmg7-K66ER163E-siRNAres-shRNAres_S | Mammalian Expression of HsSmg7-K66ER163E-siRNAres-shRNAres |
| 147463 | pCIneo-lambdaN-HA-HsSmg7-siRNAres-shRNAres_S | Mammalian Expression of HsSmg7-siRNAres-shRNAres |
| 147464 | pCIneo-lambdaN-HA-HsSmg7-T103E-siRNAres_S | Mammalian Expression of HsSmg7-T103E-siRNAres-shRNAres |
| 147465 | pCIneo-V5-SBP-CeSmg7_S | Mammalian Expression of CeSmg7 |
| 147466 | pCIneo-V5-SBP-CeSmg7-G80E_S | Mammalian Expression of CeSmg7-G80E |
| 147467 | pCIneo-V5-SBP-CeSmg7-T83E_S | Mammalian Expression of CeSmg7-T83E |
| 147468 | pGEX-6P-1-CeSMG5_1-420_S | Bacterial Expression of CeSMG5_1-420 |
| 147469 | pGEX-6P-1-CeSMG5_1-420-G91E_S | Bacterial Expression of CeSMG5_1-420-G91E |
| 147470 | pN1-HsSmg5-G120E-siRNAres-V5-SBP-MBP_S | Mammalian Expression of HsSmg5-G120E-siRNAres |
| 147471 | pN1-HsSmg5-Q123E-siRNAres-V5-SBP-MBP_S | Mammalian Expression of HsSmg5-Q123E-siRNAres |
| 147472 | pN1-HsSmg7-G100E-siRNAres-V5-SBP-MBP_S | Mammalian Expression of HsSmg7-G100E-siRNAres |
| 147473 | pN1-HsSmg7-K66ER163E-siRNAres-V5-SBP-MBP_S | Mammalian Expression of HsSmg7-K66ER163E-siRNAres |
| 147474 | pN1-HsSmg7-siRNAres-V5-SBP-MBP_S | Mammalian Expression of HsSmg7-siRNAres |
| 147475 | pRSFDuetMP-CeSmg7_1-395_S | Bacterial Expression of CeSmg7_1-395 |
| 147476 | pRSFDuetMP-CeSmg7_1-395-G80E_S | Bacterial Expression of CeSmg7_1-395-G80E |
| 147477 | pT7-V5-SBP-C1-HsUpf1_115-1129-S1107A-siRNAres_S | Mammalian Expression of HsUpf1_115-1129-S1107A-siRNAres |
| 147478 | pT7-V5-SBP-C1-HsUpf1-S1107A-siRNAres_S | Mammalian Expression of HsUpf1-S1107A-siRNAres |