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SMG6 is the catalytic endonuclease that cleaves mRNAs containing nonsense codons in metazoan.

Huntzinger E, Kashima I, Fauser M, Saulière J, Izaurralde E
RNA. 2008 Dec . 14(12):2609-17. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
146154pAc5.1C-RLuc-GFP-PTC_DInsect Expression of GFP-PTC
146155pAc5.1C-RLuc-GFP-WT_DInsect Expression of GFP-WT
146230pAc5.1B-EGFP-DmSMG6-siRNAres-V5His6_EInsect Expression of DmSMG6-siRNAres
146231pAc5.1B-lambdaN-HA-DmNOB1_EInsect Expression of DmNOB1
146232pAc5.1B-lambdaN-HA-DmSMG5-PINSMG6-V5His6_EInsect Expression of DmSMG5-PINSMG6
146233pAc5.1B-lambdaN-HA-DmSMG5-siRNAres-V5His6_EInsect Expression of DmSMG5-siRNAres
146234pAc5.1B-lambdaN-HA-DmSMG6-dellinker-siRNAres-V5His6_EInsect Expression of DmSMG6-dellinker-siRNAres
146235pAc5.1B-lambdaN-HA-DmSMG6-delPIN-siRNAres-V5His6_EInsect Expression of DmSMG6-delPIN-siRNAres
146236pAc5.1B-lambdaN-HA-DmSMG6-mut-siRNAres-V5His6_EInsect Expression of DmSMG6-mut-siRNAres
146237pAc5.1B-lambdaN-HA-DmSMG6-NusA-siRNAres-V5His6_EInsect Expression of DmSMG6-NusA-siRNAres
146238pAc5.1B-lambdaN-HA-DmSMG6-PINNOB1mut-siRNAres_EInsect Expression of DmSMG6-PINNOB1mut-siRNAres
146239pAc5.1B-lambdaN-HA-DmSMG6-PINNOB1-siRNAres_EInsect Expression of DmSMG6-PINNOB1-siRNAres
146240pAc5.1B-lambdaN-HA-DmSMG6-PINSMG5-siRNAres-V5His6_EInsect Expression of DmSMG6-PINSMG5-siRNAres
146241pAc5.1B-lambdaN-HA-DmSMG6-siRNAres-V5His6_EInsect Expression of DmSMG6-siRNAres
146291pAc5.1B-EGFP-DmSmg5-siRNAres-V5His6_FInsect Expression of DmSmg5-siRNAres
146587pCIneo-V5-SBP-HsSmg6-mut-siRNAres_IMammalian Expression of HsSmg6-mut-siRNAres
146588pEYFP-N1-HsSmg6-mut-siRNAres_IMammalian Expression of HsSmg6-mut-siRNAres

Antibodies from Article