The silencing domain of GW182 interacts with PABPC1 to promote translational repression and degradation of miRNA targets and is required for target release.
Zekri L, Huntzinger E, Heimstädt S, Izaurralde E
Mol Cell Biol. 2009 Sep 21. ():.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 145894 | pAc5.1B-lambdaN-HA-DmCCR4_A | Insect Expression of DmCCR4 |
| 145895 | pAc5.1B-lambdaN-HA-DmNot3_A | Insect Expression of DmNot3 |
| 146223 | pAc5.1B-lambdaN-HA-GST-V5His6_E | Insect Expression of GST |
| 146273 | pAc5.1A-DmNot4-EGFP_F | Insect Expression of DmNot4 |
| 146274 | pAc5.1B-lambdaN-HA-DmNot2_F | Insect Expression of DmNot2 |
| 146275 | pAc5.1B-lambdaN-HA-DmNot4_F | Insect Expression of DmNot4 |
| 146387 | pAc5.1B-lambdaN-HA-DmGW182_1-605-dsRNAres_G | Insect Expression of DmGW182_1-605-dsRNAres |
| 146388 | pAc5.1B-lambdaN-HA-DmGW182_1-830-dsRNAres_G | Insect Expression of DmGW182_1-830-dsRNAres |
| 146389 | pAc5.1B-lambdaN-HA-DmGW182_1-1115-dsRNAres_G | Insect Expression of DmGW182_1-1115-dsRNAres |
| 146390 | pAc5.1B-EGFP-DmPop2a-V5His6_G | Insect Expression of DmPop2a |
| 146391 | pAc5.1B-lambdaN-HA-DmeIF4E-siRNAres_G | Insect Expression of DmeIF4E-siRNAres |
| 146392 | pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G | Insect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies. |
| 146393 | pAc5.1B-lambdaN-HA-DmGW182-delDUFdelCterm_G | Insect Expression of DmGW182-delDUFdelCterm |
| 146394 | pAc5.1B-lambdaN-HA-DmGW182-delMGWdelRBD_G | Insect Expression of DmGW182-MGWdelRBD |
| 146395 | pAc5.1B-lambdaN-HA-DmGW182-delQdelMGW_G | Insect Expression of DmGW182-delQdelMGW |
| 146396 | pAc5.1B-lambdaN-HA-DmGW182-delUBAdelQrich_G | Insect Expression of DmGW182-delUBAdelQrich |
| 146397 | pAc5.1B-lambdaN-HA-DmPop2a-V5His6_G | Insect Expression of DmPop2a |
| 146477 | pAc5.1B-delLN-DmPABPC1-V5His6_H | Insect Expression of DmPABPC1 |
| 146478 | pAc5.1B-DmPABPC1-Nterm-V5His6_H | Insect Expression of DmPABPC1-Nterm |
| 146479 | pAc5.1B-DmPABPC1-RRM1-RRM2-V5His6_H | Insect Expression of DmPABPC1-RRM1-RRM2 |
| 146480 | pAc5.1B-EGFP-DmGW182-delM2-delDUF-delCterm_H | Insect Expression of DmGW182-delM2-delDUF-delCterm |
| 146481 | pAc5.1B-EGFP-DmGW182-DUF-M2-Cterm_H | Insect Expression of DmGW182-DUF-M2-Cterm |
| 146482 | pAc5.1B-EGFP-DmGW182-M2-Cterm_H | Insect Expression of DmGW182-M2-Cterm |
| 146483 | pAc5.1B-EGFP-DmGW182-M2-RBD-Cterm_H | Insect Expression of DmGW182-M2-RBD-Cterm |
| 146484 | pAc5.1B-EGFP-DmGW182-MGW-Cterm_H | Insect Expression of DmGW182-MGW-Cterm |
| 146485 | pAc5.1B-EGFP-DmGW182-MGW-RRM-Cterm_H | Insect Expression of DmGW182-MGW-RRM-Cterm |
| 146486 | pAc5.1B-HA-MBP_H | Insect Expression of MBP |
| 146487 | pAc5.1B-lambdaN-DmeIF4E-siRNAres-V5His6_H | Insect Expression of DmeIF4E-siRNAres |
| 146488 | pAc5.1B-lambdaN-DmPABPC1-Cterm-V5His6_H | Insect Expression of DmPABPC1-Cterm |
| 146489 | pAc5.1B-lambdaN-DmPABPC1-Nterm-V5His6_H | Insect Expression of DmPABPC1-Nterm |
| 146490 | pAc5.1B-lambdaN-DmPABPC1-RRM1-RRM2-V5His6_H | Insect Expression of DmPABPC1-RRM1-RRM2 |
| 146491 | pAc5.1B-lambdaN-DmPABPC1-RRM2-RRM3-V5His6_H | Insect Expression of DmPABPC1-RRM2-RRM3 |
| 146492 | pAc5.1B-lambdaN-DmPABPC1-RRM3-RRM4-V5His6_H | Insect Expression of DmPABPC1-RRM3-RRM4 |
| 146493 | pAc5.1B-lambdaN-HA-DmeIF4Ga-Nterm_H | Insect Expression of DmeIF4Ga-Nterm |
| 146494 | pAc5.1B-lambdaN-HA-DmGW182-DUF-M2-Cterm_H | Insect Expression of DmGW182-DUF-M2-Cterm |
| 146495 | pAc5.1B-lambdaN-HA-DmGW182-M2-Cterm_H | Insect Expression of DmGW182-M2-Cterm |
| 146496 | pAc5.1B-lambdaN-HA-DmGW182-M2-RBD-Cterm_H | Insect Expression of DmGW182-M2-RBD-Cterm |
| 146497 | pAc5.1B-lambdaN-HA-DmGW182-MGW-Cterm_H | Insect Expression of DmGW182-MGW-Cterm |
| 146498 | pAc5.1B-lambdaN-HA-DmGW182-MGW-RRM-Cterm_H | Insect Expression of DmGW182-MGW-RRM-Cterm |
| 146499 | pAc5.1B-lambdaN-HA-DmGW182-V5His6_H | Insect Expression of DmGW182 |