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Mapping Interactions among Cell-Free Expressed Zika Virus Proteins.

Varasteh Moradi S, Gagoski D, Mureev S, Walden P, McMahon KA, Parton RG, Johnston WA, Alexandrov K
J Proteome Res. 2020 Apr 3;19(4):1522-1532. doi: 10.1021/acs.jproteome.9b00771. Epub 2020 Mar 9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
145339pmCellFree_KA1_ZIKVCCell-free/mammalian expression vector for Zika virus capsid protein with N-term eGFP
145340pmCellFree_KA1_ZIKV PrMCell-free/mammalian expression vector for Zika virus PrM protein with N-term eGFP
145341pmCellFree_KA1 ZIKV ECell-free/mammalian expression vector for Zika virus Envelope protein with N-term eGFP
145342pmCellFree_KA1_ZIKV NS1Cell-free/mammalian expression vector for Zika virus NS1 protein with N-term eGFP
145343pmCellFree_KA1_ZIKV NS2ACell-free/mammalian expression vector for Zika virus NS2A protein with N-term eGFP
145344pmCellFree_KA1_ZIKV NS2BCell-free/ mammalian expression vector for Zika virus NS2B protein with N-term eGFP
145345pmCellFree_KA1_ZIKV NS3Cell-free/ mammalian expression vector for Zika virus NS3 protein with N-term eGFP
145346pmCellFree_KA1_ZIKV NS4ACell-free/mammalian expression vector for Zika virus NS4A protein with N-term eGFP
145347pmCellFree_KA1_ZIKV NS4BCell-free/ mammalian expression vector for Zika virus NS4B protein with N-term eGFP
145348pmCellFree_KA1_ZIKV NS5Cell-free/ mammalian expression vector for Zika virus NS5 protein with N-term eGFP
145349pCellFree_G06_ZIKVCCell-free expression vector for Zika virus Capsid protein with C-term mCherry
145350pCellFree_G06_ZIKV PrMCell-free expression vector for Zika virus prM protein with C-term mCherry
145351pCellFree_G06_ZIKV ECell-free expression vector for Zika virus Envelope protein with C-term mCherry
145352pCellFree_G06_ZIKV NS1Cell-free expression vector for Zika virus NS1 protein with C-term mCherry
145353pCellFree_G06_ZIKV NS2ACell-free expression vector for Zika virus NS2A protein with C-term mCherry
145354pCellFree_G06_ZIKV NS2BCell-free expression vector for Zika virus NS2B protein with C-term mCherry
145355pCellFree_G06_ZIKV NS3Cell-free expression vector for Zika virus NS3 protein with C-term mCherry
145356pCellFree_G06_ZIKV NS4ACell-free expression vector for Zika virus NS4A protein with C-term mCherry
145357pCellFree_G06_ZIKV NS4BCell-free expression vector for Zika virus NS4B protein with C-term mCherry
145358pCellFree_G06_ZIKV NS5Cell-free expression vector for Zika virus NS5 protein with C-term mCherry
145359pmCellFree_KA2_ZIKV CCell-free/mammalian expression vector for Zika virus capsid protein with N-term mCherry
145360pmCellFree_KA2_ZIKV PrMCell-free/mammalian expression vector for Zika virus PrM protein with N-term mCherry
145361pmCellFree_KA2_ZIKV ECell-free/mammalian expression vector for Zika virus Envelope protein with N-term mCherry
145362pmCellFree_KA2_ZIKV NS1Cell-free/mammalian expression vector for Zika virus NS1 protein with N-term mCherry
145363pmCellFree_KA2_ZIKV NS2ACell-free/mammalian expression vector for Zika virus NS2A protein with N-term mCherry
145364pmCell-Free_KA2_ZIKV NS2BCell-free/mammalian expression vector for Zika virus NS2B protein with N-term mCherry
145365pmCellFree_KA2_ZIKV NS3Cell-free/mammalian expression vector for Zika virus NS3 protein with N-term mCherry
145366pmCellFree_KA2_ZIKV NS4ACell-free/mammalian expression vector for Zika virus NS4A protein with N-term mCherry
145367pmCellFree_KA2_ZIKV NS4BCell-free/mammalian expression vector for Zika virus NS4Bprotein with N-term mCherry
145368pmCellFree _KA2_ZIKV NS5Cell-free/mammalian expression vector for Zika virus NS5 protein with N-term mCherry
145369pmCellFree_KA1Gateway destination vector for cell-free and mamalian expression with N-term 8xHis and eGFP tag
145370pmCellFree_KA2Gateway destination vector for cell-free and mamalian expression with N-term 8xHis-tag and mCherry tag

Antibodies from Article