Unblending of Transcriptional Condensates in Human Repeat Expansion Disease.
Basu S, Mackowiak SD, Niskanen H, Knezevic D, Asimi V, Grosswendt S, Geertsema H, Ali S, Jerkovic I, Ewers H, Mundlos S, Meissner A, Ibrahim DM, Hnisz D
Cell. 2020 May 28;181(5):1062-1079.e30. doi: 10.1016/j.cell.2020.04.018. Epub 2020 May 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 145228 | pGAL4-DBD-BHLHE41-Adel-IDR | Expresses fusion of GAL4 DNA-binding domain and BHLHE41-Adel IDR |
| 145229 | pGAL4-DBD-BHLHE41wt-IDR | Expresses fusion of GAL4 DNA-binding domain and BHLHE41wt IDR |
| 145230 | pGAL4-DBD-EOMES-Adel-IDR | Expresses fusion of GAL4 DNA-binding domain and EOMES-Adel IDR |
| 145231 | pGAL4-DBD-EOMESwt-IDR | Expresses fusion of GAL4 DNA-binding domain and EOMESwt IDR |
| 145232 | pGAL4-DBD-GSX2-Adel-IDR | Expresses fusion of GAL4 DNA-binding domain and GSX2-Adel IDR |
| 145233 | pGAL4-DBD-GSX2wt-IDR | Expresses fusion of GAL4 DNA-binding domain and GSX2wt IDR |
| 145234 | pGAL4-DBD-HEY2-Adel-IDR | Expresses fusion of GAL4 DNA-binding domain and HEY2-Adel IDR |
| 145235 | pGAL4-DBD-HEY2wt-IDR | Expresses fusion of GAL4 DNA-binding domain and HEY2wt IDR |
| 145236 | pGAL4-DBD-HOXA13-15A-IDR | Expresses fusion of GAL4 DNA-binding domain and HOXA13-15A IDR |
| 145237 | pGAL4-DBD-HOXA13-44A-IDR | Expresses fusion of GAL4 DNA-binding domain and HOXA13-44A IDR |
| 145238 | pGAL4-DBD-HOXA13-58A-IDR | Expresses fusion of GAL4 DNA-binding domain and HOXA13-58A IDR |
| 145239 | pGAL4-DBD-HOXA13+7A-IDR | Expresses fusion of GAL4 DNA-binding domain and HOXA13+7A IDR |
| 145240 | pGAL4-DBD-HOXA13wt-IDR | Expresses fusion of GAL4 DNA-binding domain and HOXA13wt IDR |
| 145241 | pGAL4-DBD-HOXD11-Adel-IDR | Expresses fusion of GAL4 DNA-binding domain and HOXD11-Adel IDR |
| 145242 | pGAL4-DBD-HOXD11wt-IDR | Expresses fusion of GAL4 DNA-binding domain and HOXD11wt-IDR |
| 145243 | pGAL4-DBD-HOXD13-Adel-15A-IDR | Expresses fusion of GAL4 DNA-binding domain and HOXD13-Adel-15A IDR |
| 145244 | pGAL4-DBD-HOXD13wt-IDR | Expresses fusion of GAL4 DNA-binding domain and HOXD13wt IDR |
| 145245 | pGAL4-DBD-MCS | Backbone vector expressing GAL4 DNA-binding domain followed by MCS |
| 145246 | pGAL4-DBD-MCS-long | Backbone vector expressing GAL4 DNA-binding domain followed by MCS |
| 145247 | pGAL4-DBD-MNX1-Adel-IDR | Expresses fusion of GAL4 DNA-binding domain and MNX1-Adel IDR |
| 145248 | pGAL4-DBD-MNX1wt-IDR | Expresses fusion of GAL4 DNA-binding domain and MNX1wt IDR |
| 145249 | pGAL4-DBD-OLIG2-Adel-IDR | Expresses fusion of GAL4 DNA-binding domain and OLIG2-Adel IDR |
| 145250 | pGAL4-DBD-OLIG2wt-IDR | Expresses fusion of GAL4 DNA-binding domain and OLIG2wt IDR |
| 145251 | pGAL4-DBD-RUNX2+10A-IDR | Expresses fusion of GAL4 DNA-binding domain and RUNX2A+10A IDR |
| 145252 | pGAL4-DBD-RUNX2wt-IDR | Expresses fusion of GAL4 DNA-binding domain and RUNX2Awt IDR |
| 145253 | pGAL4-DBD-TBX1-Adel-IDR | Expresses fusion of GAL4 DNA-binding domain and TBX1-Adel IDR |
| 145254 | pGAL4-DBD-TBX1wt-IDR | Expresses fusion of GAL4 DNA-binding domain and TBX1wt IDR |
| 145255 | pCFP-LacI-HOXD13wt-IDR | Expresses fusion of ECFP-LacI and HOXD13wt IDR |
| 145256 | pCFP-LacI-HOXD13(+7A)-IDR | Expresses fusion of ECFP-LacI and HOXD13+7A IDR |
| 145257 | pCFP-LacI-HOXD13(+10A)-IDR | Expresses fusion of ECFP-LacI and HOXD13+10A IDR |
| 145258 | pHR-HOXD13-IDR-mCherry-CRY2 | Mammalian Expression of Opto HOXD13 IDR |
| 145259 | pHR-mCherry-CRY2-NLS | Mammalian Expression of Nuclear Control OptoConstruct with SV40 NLS |
| 145260 | pHR-HOXD13-IDR-mCherry-CRY2-NLS | Mammalian Expression of Nuclear Opto HOXD13 IDR with SV40 NLS |
| 145261 | pHR-HOXD13(+7A)-IDR-mCherry-CRY2-NLS | Mammalian Expression of Nuclear Opto HOXD13(+7A) IDR with SV40 NLS |
| 145262 | pHR-HOXD13(+8A)-IDR-mCherry-CRY2-NLS | Mammalian Expression of Nuclear Opto HOXD13(+8A) IDR with SV40 NLS |
| 145263 | pHR-HOXD13(+9A)-IDR-mCherry-CRY2-NLS | Mammalian Expression of Nuclear Opto HOXD13(+9A) IDR with SV40 NLS |
| 145265 | pHR-HOXA13-IDR-mCherry-CRY2-NLS | Mammalian Expression of Nuclear Opto HOXA13 IDR with SV40 NLS |
| 145266 | pHR-HOXA13(+7A)-IDR-mCherry-CRY2-NLS | Mammalian Expression of Nuclear Opto HOXA13(+7A) IDR with SV40 NLS |
| 145267 | pHR-RUNX2-IDR-mCherry-CRY2-NLS | Mammalian Expression of Nuclear Opto RUNX2 IDR with SV40 NLS |
| 145268 | pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS | Mammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLS |
| 145269 | pHR-TBP(38Q)-IDR-mCherry-Cry2-NLS | Mammalian Expression of Nuclear Opto TBP(38Q) IDR with SV40 NLS |
| 145270 | pHR-TBP(53Q)-IDR-mCherry-Cry2-NLS | Mammalian Expression of Nuclear Opto TBP(53Q) IDR with SV40 NLS |
| 145271 | pHR-HOXD13(-7A)-IDR-mCherry-CRY2-NLS | Mammalian Expression of Nuclear Opto HOXD13(-7A) IDR with SV40 NLS |
| 145272 | pHR-HOXD13(-15A)-IDR-mCherry-CRY2-NLS | Mammalian Expression of Nuclear Opto HOXD13(-15A) IDR with SV40 NLS |
| 145273 | pHR-HOXD13(DEdel)-IDR-mCherry-CRY2-NLS | Mammalian Expression of Nuclear Opto HOXD13(DEdel) IDR with SV40 NLS |
| 145274 | pHR-MCS-YFP-NLS | Mammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLS |
| 145275 | pHR-MED1trunc-IDR-YFP-NLS | Mammalian Expression of Nuclear MED1 truncated IDR-YFP with SV40 NLS |
| 145276 | pHR-MCS-MED1long-IDR-YFP-NLS | Mammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLS |
| 145277 | pHR-HOXD13-IDR-YFP-NLS | Mammalian Expression of Nuclear HOXD13 IDR-YFP with SV40 NLS |
| 145278 | pHR-HOXD13(+7A)-IDR-YFP-NLS | Mammalian Expression of Nuclear HOXD13 (+7A) IDR-YFP with SV40 NLS |
| 145279 | pET-45b-mCherry | Bacterial Expression of 6xHis mCherry Fusion Protein |
| 145280 | pET-45b-HOXD13-IDR-mCherry | Bacterial Expression of 6xHis HOXD13 IDR mCherry Fusion Protein |
| 145281 | pET-45b-HOXD13(+7A)-IDR-mCherry | Bacterial Expression of 6xHis HOXD13 (+7A) IDR mCherry Fusion Protein |
| 145282 | pET-45b-HOXD13(+10A)-IDR-mCherry | Bacterial Expression of 6xHis HOXD13 (+10A) IDR mCherry Fusion Protein |
| 145283 | pET-45b-TBP(38Q)-IDR-mCherry | Bacterial Expression of 6xHis TBP(38Q) IDR mCherry Fusion Protein |
| 145284 | pET-45b-HOXA13(WT)-IDR-mCherry | Bacterial Expression of 6xHis HOXA13(WT) IDR mCherry Fusion Protein |
| 145285 | pET-45b-HOXA13(+7A)-IDR-mCherry | Bacterial Expression of 6xHis HOXA13(+7A)IDR mCherry Fusion Protein |
| 145286 | pET-45b-RUNX2(WT)-IDR-mCherry | Bacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion Protein |
| 145287 | pET-45b-RUNX2(+10A)-IDR-mCherry | Bacterial Expression of 6xHis RUNX2(+10A) IDR mCherry Fusion Protein |
| 145288 | RCASBP(A)-3xFlag-mHoxd13wt | Avian Expression of murine wildtype Hoxd13 with N-terminal 3xFlag tag |
| 145289 | RCASBP(A)-3xFlag-mHoxd13+7Ala | Avian Expression of murine SPDH (+7Ala) Hoxd13 with N-terminal 3xFlag tag |