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CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos.

Kushawah G, Hernandez-Huertas L, Abugattas-Nunez Del Prado J, Martinez-Morales JR, DeVore ML, Hassan H, Moreno-Sanchez I, Tomas-Gallardo L, Diaz-Moscoso A, Monges DE, Guelfo JR, Theune WC, Brannan EO, Wang W, Corbin TJ, Moran AM, Sanchez Alvarado A, Malaga-Trillo E, Takacs CM, Bazzini AA, Moreno-Mateos MA
Dev Cell. 2020 Sep 28;54(6):805-817.e7. doi: 10.1016/j.devcel.2020.07.013. Epub 2020 Aug 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
141320pT3TS-RfxCas13d-HAPlasmid to carry out IVT of RfxCas13d (human codon-optimized)
141321pT3TS-RfxCas13d-NLS-HAPlasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)
141322pET-28b-RfxCas13d-HisPlasmid for bacterial expression and purification of RfxCas13d protein

Antibodies from Article