Expanding the bioluminescent reporter toolkit for plant science with NanoLUC.
Urquiza-Garcia U, Millar AJ
Plant Methods. 2019 Jul 8;15:68. doi: 10.1186/s13007-019-0454-4. eCollection 2019.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 141285 | pGWB401NL3F10H | No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, KmR for plant) |
| 141286 | pGWB501NL3F10H | No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, HygR for plant) |
| 141287 | pGWB601NL3F10H | No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, BASTA R for plant) |
| 141288 | pGWB701NL3F10H | No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant) |
| 141289 | pET-28a(+)::NL | NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR) |
| 141290 | pET28a(+)::NL3F10H | NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR) |
| 141291 | pET28a(+)::MBP-NL3F10H | MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR) |