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A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.

Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale M, Raught B, Jacob Y, Roth FP
G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 10.1534/g3.120.401554. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
141255pDONR207 SARS-CoV-2 NSP1Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141256pDONR223 SARS-CoV-2 NSP2Gateway-compatible Entry vector, with insert of NSP2 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141257pDONR207 SARS-CoV-2 NSP3Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141258pDONR223 SARS-CoV-2 NSP4Gateway-compatible Entry vector, with insert of NSP4 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141259pDONR223 SARS-CoV-2 NSP5Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141260pDONR223 SARS-CoV-2 NSP6Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141261pDONR223 SARS-CoV-2 NSP7Gateway-compatible Entry vector, with insert of NSP7 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141262pDONR223 SARS-CoV-2 NSP8Gateway-compatible Entry vector, with insert of NSP8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141263pDONR223 SARS-CoV-2 NSP9Gateway-compatible Entry vector, with insert of NSP9 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141264pDONR223 SARS-CoV-2 NSP10Gateway-compatible Entry vector, with insert of NSP10 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141265pDONR223 SARS-CoV-2 NSP12Gateway-compatible Entry vector, with insert of NSP12 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141266pDONR223 SARS-CoV-2 NSP13Gateway-compatible Entry vector, with insert of NSP13 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141267pDONR223 SARS-CoV-2 NSP14Gateway-compatible Entry vector, with insert of NSP14 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141268pDONR223 SARS-CoV-2 NSP15Gateway-compatible Entry vector, with insert of NSP15 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141269pDONR223 SARS-CoV-2 NSP16Gateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141271pDONR207 SARS-CoV-2 ORF3AGateway-compatible Entry vector, with insert of ORF3A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141272pDONR223 SARS-CoV-2 ORF3BGateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141273pDONR207 SARS-CoV-2 EGateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141274pDONR207 SARS-CoV-2 MGateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141275pDONR223 SARS-CoV-2 ORF6Gateway-compatible Entry vector, with insert of ORF6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141276pDONR207 SARS-CoV-2 ORF7AGateway-compatible Entry vector, with insert of ORF7A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141277pDONR223 SARS-CoV-2 ORF7BGateway-compatible Entry vector, with insert of ORF7B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141278pDONR223 SARS-CoV-2 ORF8Gateway-compatible Entry vector, with insert of ORF8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
141280pDONR223 SARS-CoV-2 ORF9BGateway-compatible Entry vector, with insert of ORF9B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
149304pDONR207 SARS-CoV-2 NSP1_nostopGateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149305pDONR223 SARS-CoV-2 NSP2_nostopGateway-compatible Entry vector, with insert of NSP2 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149306pDONR207 SARS-CoV-2 NSP3_nostopGateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149307pDONR223 SARS-CoV-2 NSP4_nostopGateway-compatible Entry vector, with insert of NSP4 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149308pDONR223 SARS-CoV-2 NSP5_nostopGateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149309pDONR223 SARS-CoV-2 NSP6_nostopGateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149310pDONR223 SARS-CoV-2 NSP7_nostopGateway-compatible Entry vector, with insert of NSP7 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149311pDONR223 SARS-CoV-2 NSP8_nostopGateway-compatible Entry vector, with insert of NSP8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149312pDONR223 SARS-CoV-2 NSP9_nostopGateway-compatible Entry vector, with insert of NSP9 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149313pDONR223 SARS-CoV-2 NSP10_nostopGateway-compatible Entry vector, with insert of NSP10 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149314pDONR223 SARS-CoV-2 NSP12_nostopGateway-compatible Entry vector, with insert of NSP12 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149315pDONR223 SARS-CoV-2 NSP13_nostopGateway-compatible Entry vector, with insert of NSP13 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149316pDONR223 SARS-CoV-2 NSP14_nostopGateway-compatible Entry vector, with insert of NSP14 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149317pDONR223 SARS-CoV-2 NSP15_nostopGateway-compatible Entry vector, with insert of NSP15 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149318pDONR223 SARS-CoV-2 NSP16_nostopGateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149319pDONR207 SARS-CoV-2 ORF3A_nostopGateway-compatible Entry vector, with insert of ORF3A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149320pDONR223 SARS-CoV-2 ORF3B_nostopGateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149321pDONR207 SARS-CoV-2 E_nostopGateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149322pDONR207 SARS-CoV-2 M_nostopGateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149323pDONR223 SARS-CoV-2 ORF6_nostopGateway-compatible Entry vector, with insert of ORF6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149324pDONR207 SARS-CoV-2 ORF7A_nostopGateway-compatible Entry vector, with insert of ORF7A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149325pDONR223 SARS-CoV-2 ORF7B_nostopGateway-compatible Entry vector, with insert of ORF7B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149326pDONR223 SARS-CoV-2 ORF8_nostopGateway-compatible Entry vector, with insert of ORF8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149327pDONR223 SARS-CoV-2 ORF9B_nostopGateway-compatible Entry vector, with insert of ORF9B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
149328pDONR223 SARS-CoV-2 ORF9Bwu_nostopGateway-compatible Entry vector, with insert of ORF9Bwu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020. Does not contain stop codon.
149329pDONR223 SARS-CoV-2 SGateway-compatible Entry vector, with insert of the spike (S) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
149330pDONR223 SARS-CoV-2 NGateway-compatible Entry vector, with insert of the nucleocapsid (N) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
149331pDONR223 SARS-CoV-2 ORF9BwuGateway-compatible Entry vector, with insert of ORF9Bwu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020
149332pDONR223 SARS-CoV-2 ORF10wuGateway-compatible Entry vector, with insert of ORF10wu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020
152986pDONR223 SARS-CoV-2 ORF10wu_nostop Gateway-compatible Entry vector, with insert of ORF10wu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020
152987pDONR223 SARS-CoV-2 N_nostopGateway-compatible Entry vector, with insert of N gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
152988pDONR223 SARS-CoV-2 S_nostop Gateway-compatible Entry vector, with insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
153173pDONR221 SARS-CoV-2 S-frag1_nostopGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
153174pDONR221 SARS-CoV-2 S-frag2_nostopGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
153175pDONR221 SARS-CoV-2 S-frag3_nostopGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
153176pDONR221 SARS-CoV-2 S-frag4_nostopGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
153177pDONR221 SARS-CoV-2 S-frag5_nostopGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
153184pDONR221 SARS-CoV-2 S-frag6_nostopGateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
153952pDONR223 SARS-CoV-2 S 24nt-delGateway-compatible Entry vector, with insert of S CDS bearing a 24nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020
153953pDONR223 SARS-CoV-2 S 24nt-del_nostopGateway-compatible Entry vector, with insert of S CDS bearing a 24nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020
153954pDONR207 SARS-CoV-2 E 27nt-delGateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020
153955pDONR207 SARS-CoV-2 E 27nt-del_nostopGateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020
153956pDONR223 SARS-CoV-2 ORF7B C-trunc_nostopGateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020
153957pDONR223 SARS-CoV-2 ORF7B C-truncGateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020
154027pDONR207 SARS-CoV-2 NSP3 Cys857AlaGateway-compatible Entry vector
154028pDONR223 SARS-CoV-2 NSP5 Cys146AlaGateway-compatible Entry vector
154029pDONR207 SARS-CoV-2 NSP3 Cys857Ala_nostopGateway-compatible Entry vector
154030pDONR223 SARS-CoV-2 NSP5 Cys146Ala_nostopGateway-compatible Entry vector
154400pDONR223 SARS-CoV2 TEV-NSP1Gateway-compatible Entry vector
154401pDONR223 SARS-CoV2 TEV-NSP2Gateway-compatible Entry vector
154402pDONR207 SARS-CoV2 TEV-NSP3Gateway-compatible Entry vector
154403pDONR223 SARS-CoV2 TEV-NSP4Gateway-compatible Entry vector
154404pDONR223 SARS-CoV2 TEV-NSP5Gateway-compatible Entry vector
154405pDONR223 SARS-CoV2 TEV-NSP6Gateway-compatible Entry vector
154406pDONR223 SARS-CoV2 TEV-NSP7Gateway-compatible Entry vector
154407pDONR223 SARS-CoV2 TEV-NSP8Gateway-compatible Entry vector
154408pDONR223 SARS-CoV2 TEV-NSP9Gateway-compatible Entry vector
154409pDONR223 SARS-CoV2 TEV-NSP10Gateway-compatible Entry vector
154410pDONR223 SARS-CoV2 TEV-NSP12Gateway-compatible Entry vector
154411pDONR223 SARS-CoV2 TEV-NSP13Gateway-compatible Entry vector
154412pDONR223 SARS-CoV2 TEV-NSP14Gateway-compatible Entry vector
154413pDONR223 SARS-CoV2 TEV-NSP15Gateway-compatible Entry vector
154414pDONR223 SARS-CoV2 TEV-NSP16Gateway-compatible Entry vector
154415pDONR223 SARS-CoV2 TEV-SGateway-compatible Entry vector
154416pDONR223 SARS-CoV2 TEV-ORF3AGateway-compatible Entry vector
154417pDONR223 SARS-CoV2 TEV-ORF3BGateway-compatible Entry vector
154418pDONR223 SARS-CoV2 TEV-EGateway-compatible Entry vector
154419pDONR223 SARS-CoV2 TEV-MGateway-compatible Entry vector
154420pDONR223 SARS-CoV2 TEV-ORF6Gateway-compatible Entry vector
154421pDONR223 SARS-CoV2 TEV-ORF7AGateway-compatible Entry vector
154422pDONR223 SARS-CoV2 TEV-ORF7BGateway-compatible Entry vector
154423pDONR223 SARS-CoV2 TEV-ORF8Gateway-compatible Entry vector
154424pDONR223 SARS-CoV2 TEV-NGateway-compatible Entry vector
154425pDONR223 SARS-CoV2 TEV-ORF9BGateway-compatible Entry vector

Antibodies from Article