Preloading budding yeast with all-in-one CRISPR/Cas9 vectors for easy and high-efficient genome editing.

Degreif D, Kremenovic M, Geiger T, Bertl A
J Biol Methods. 2018 Sep 12;5(3):e98. doi: 10.14440/jbm.2018.254. eCollection 2018. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
141249	pCAS9i	Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
141250	pCAS9c	Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
141251	pCAS9id	Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
141252	pCAS9cd	Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.