Enhancing PLP-Binding Capacity of Class-III omega-Transaminase by Single Residue Substitution.
Roura Padrosa D, Alaux R, Smith P, Dreveny I, Lopez-Gallego F, Paradisi F
Front Bioeng Biotechnol. 2019 Oct 18;7:282. doi: 10.3389/fbioe.2019.00282. eCollection 2019.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
141207 | pMP89b CvTA V124N | Chromobacterium violaceum transaminase with enhances affinity for PLP. TEV cleaving site after the N-terminal Histag. |