Engineering human ACE2 to optimize binding to the spike protein of SARS coronavirus 2.
Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert AS, Procko E
Science. 2020 Sep 4;369(6508):1261-1265. doi: 10.1126/science.abc0870. Epub 2020 Aug 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 141183 | pcDNA3-SARS-CoV-2-S-RBD-Fc | Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc |
| 141184 | pcDNA3-SARS-CoV-2-S-RBD-sfGFP | Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP |
| 141185 | pCEP4-myc-ACE2 | Mammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tag |
| 145145 | pcDNA3-SARS-CoV-2-S-RBD-8his | Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag |
| 145146 | pcDNA3-J chain | Mammalian expression plasmid for J chain of polymeric antibody classes |
| 145147 | pcDNA3-sACE2(WT) | Mammalian expression plasmid for soluble ACE2 (protease domain) |
| 145148 | pcDNA3-sACE2v1 | Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 1 |
| 145149 | pcDNA3-sACE2v2 | Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 2 |
| 145150 | pcDNA3-sACE2v3 | Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 3 |
| 145151 | pcDNA3-sACE2v4 | Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 4 |
| 145152 | pcDNA3-sACE2v5 | Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 5 |
| 145153 | pcDNA3-sACE2v6 | Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 6 |
| 145154 | pcDNA3-sACE2(WT)-Fc(IgA1) | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) |
| 145155 | pcDNA3-sACE2v1-Fc(IgA1) | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 1 |
| 145156 | pcDNA3-sACE2-T92Q | Mammalian expression plasmid for soluble ACE2 (protease domain) T92Q glycosylation mutant |
| 145157 | pcDNA3-sACE2v2-Fc(IgA1) | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 2 |
| 145158 | pcDNA3-sACE2v3-Fc(IgA1) | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 3 |
| 145159 | pcDNA3-sACE2v4-Fc(IgA1) | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 4 |
| 145160 | pcDNA3-sACE2v5-Fc(IgA1) | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 5 |
| 145161 | pcDNA3-sACE2v6-Fc(IgA1) | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 6 |
| 145162 | pcDNA3-sACE2-T92Q-Fc(IgA1) | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) glycosylation mutant T92Q |
| 145163 | pcDNA3-sACE2(WT)-Fc(IgG1) | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) |
| 145164 | pcDNA3-sACE2v1-Fc(IgG1) | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity variant 1 |
| 145165 | pcDNA3-sACE2v2-Fc(IgG1) | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2 |
| 145166 | pcDNA3-sACE2v3-Fc(IgG1) | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 3 |
| 145167 | pcDNA3-sACE2v4-Fc(IgG1) | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 4 |
| 145168 | pcDNA3-sACE2v5-Fc(IgG1) | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 5 |
| 145169 | pcDNA3-sACE2v6-Fc(IgG1) | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 6 |
| 145170 | pcDNA3-sACE2-T92Q-Fc(IgG1) | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) glycosylation mutant T92Q |
| 145171 | pcDNA3-sACE2(WT)-sfGFP | Mammalian expression plasmid for soluble ACE2-super folder GFP fusion |
| 145172 | pcDNA3-sACE2v1-sfGFP | Mammalian expression plasmid for sACE2-sfGFP high affinity variant 1 |
| 145173 | pcDNA3-sACE2v2-sfGFP | Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2 |
| 145174 | pcDNA3-sACE2v3-sfGFP | Mammalian expression plasmid for sACE2-sfGFP high affinity variant 3 |
| 145175 | pcDNA3-sACE2v4-sfGFP | Mammalian expression plasmid for sACE2-sfGFP high affinity variant 4 |
| 145176 | pcDNA3-sACE2v5-sfGFP | Mammalian expression plasmid for sACE2-sfGFP high affinity variant 5 |
| 145177 | pcDNA3-sACE2v6-sfGFP | Mammalian expression plasmid for sACE2-sfGFP high affinity variant 6 |
| 145178 | pcDNA3-sACE2-T92Q-sfGFP | Mammalian expression plasmid for sACE2-sfGFP glycosylation mutant T92Q |
| 149268 | pcDNA3-sACE2(WT)-8his | Mammalian expression plasmid for his-tagged sACE2 |
| 149269 | pcDNA3-sACE2v1-8his | Mammalian expression plasmid for his-tagged sACE2 high affinity variant 1 |
| 149270 | pcDNA3-sACE2v2-8his | Mammalian expression plasmid for his-tagged sACE2 high affinity variant 2 |
| 149271 | pcDNA3-sACE2v3-8his | Mammalian expression plasmid for his-tagged sACE2 high affinity variant 3 |
| 149663 | pcDNA3-sACE2v2.2-8his | Mammalian expression plasmid for his-tagged sACE2 high affinity variant 2.2 |
| 149664 | pcDNA3-sACE2v2.4-8his | Mammalian expression plasmid for his-tagged sACE2 high affinity variant 2.4 |
| 149665 | pcDNA3-sACE2v2.2-sfGFP | Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2.2 |
| 149666 | pcDNA3-sACE2v2.4-sfGFP | Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2.4 |
| 149667 | pcDNA3-sACE2v2.2-IgG1 | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2.2 |
| 149668 | pcDNA3-sACE2v2.4-IgG1 | Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2.4 |
| 154098 | pcDNA3-sACE2-WT(732) | Mammalian expression plasmid for dimeric soluble ACE2 (untagged) |
| 154099 | pcDNA3-sACE2v2(732) | Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (untagged) |
| 154100 | pcDNA3-sACE2v2.4(732) | Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (untagged) |
| 154101 | pcDNA3-sACE2-WT(732)-8h | Mammalian expression plasmid for dimeric soluble ACE2 (8his tag) |
| 154102 | pcDNA3-sACE2v2(732)-8h | Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (8his tag) |
| 154103 | pcDNA3-sACE2v2.4(732)-8h | Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag) |
| 154104 | pcDNA3-sACE2-WT(732)-IgG1 | Mammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion) |
| 154105 | pcDNA3-sACE2v2(732)-IgG1 | Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion) |
| 154106 | pcDNA3-sACE2v2.4(732)-IgG1 | Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (IgG1-Fc fusion) |