TRUPATH, an open-source biosensor platform for interrogating the GPCR transducerome.
Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT
Nat Chem Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 10.1038/s41589-020-0535-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140973 | pcDNA5/FRT/TO-GAlphai1-RLuc8 | Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140974 | pcDNA5/FRT/TO-GAlphai2-RLuc8 | Encodes a G alpha subunit (GNAI2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140975 | pcDNA5/FRT/TO-GAlphai3-RLuc8 | Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140976 | pcDNA5/FRT/TO-GAlphaoA-RLuc8 | Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140977 | pcDNA5/FRT/TO-GAlphaoB-RLuc8 | Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140978 | pcDNA5/FRT/TO-GAlphaz-RLuc8 | Encodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140979 | pcDNA5/FRT/TO-GAlphaGustducin-RLuc8 | Encodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140980 | pcDNA5/FRT/TO-GAlphasS-RLuc8 | Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140981 | pcDNA5/FRT/TO-GAlphasL-RLuc8 | Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140982 | pcDNA5/FRT/TO-GAlphaQ-RLuc8 | Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140983 | pcDNA5/FRT/TO-GAlpha11-RLuc8 | Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140984 | pcDNA5/FRT/TO-GAlpha15-RLuc8 | Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140985 | pcDNA5/FRT/TO-GAlpha12-RLuc8 | Encodes a G alpha subunit (GNA12) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140986 | pcDNA5/FRT/TO-GAlpha13-RLuc8 | Encodes a G alpha subunit (GNA13) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140987 | pcDNA3.1-Beta1 | Encodes a Gbeta subunit (GNB1) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140988 | pcDNA3.1-Beta3 | Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140989 | pcDNA3.1-GGamma1-GFP2 | Encodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140990 | pcDNA3.1-GGamma8-GFP2 | Encodes a G gamma subunit (GNG8) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140991 | pcDNA3.1-GGamma9-GFP2 | Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 140992 | pcDNA3.1-GGamma13-GFP2 | Encodes a G gamma subunit (GNG13) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 166772 | pcDNA3.1-Beta2 | Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 166773 | pcDNA3.1-Beta4 | Encodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 166774 | pcDNA3.1-GGamma2-GFP2 | Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 166775 | pcDNA3.1-GGamma3-GFP2 | Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 166776 | pcDNA3.1-GGamma4-GFP2 | Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 166777 | pcDNA3.1-GGamma5-GFP2 | Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 166778 | pcDNA3.1-GGamma7-GFP2 | Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 166779 | pcDNA3.1-GGamma10-GFP2 | Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 166780 | pcDNA3.1-GGamma11-GFP2 | Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 166781 | pcDNA3.1-GGamma12-GFP2 | Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation |
| 196048 | TRUPATH Triple Gai1 | Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins |
| 196049 | TRUPATH Triple Gai2 | Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins |
| 196050 | TRUPATH Triple Gai3 | Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins |
| 196051 | TRUPATH Triple GaoA | Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor |
| 196052 | TRUPATH Triple GaoB | Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor |
| 196053 | TRUPATH Triple GaZ | Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins |
| 196054 | TRUPATH Triple Gagust | Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins |
| 196055 | TRUPATH Triple (bidirectional) GasS | Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins |
| 196058 | TRUPATH Triple Gaq | Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins |
| 196059 | TRUPATH Triple Ga11 | Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins |
| 196060 | TRUPATH Triple Ga15 | Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins |
| 196061 | TRUPATH Triple Ga12 | Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins |
| 196062 | TRUPATH Triple Ga13 | Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins |