Multivalent interactions between CsoS2 and Rubisco mediate alpha-carboxysome formation.
Oltrogge LM, Chaijarasphong T, Chen AW, Bolin ER, Marqusee S, Savage DF
Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 10.1038/s41594-020-0387-7. Epub 2020 Mar 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140839 | pBz15_Strep_Rubisco | wild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backbone |
| 140840 | pLz74_Rubisco_CsoS2_Npeptide_fusion | Same as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallization |
| 140841 | pBz118_His_CsoS2 | Halothiobacillus neapolitanus CsoS2 with N-terminal His-tag, pET-14b backbone |
| 140842 | pBz109_CsoS2_NTD | His-tagged CsoS2 N-terminal domain (NTD), pET-14b backbone |
| 140843 | pBz106_CsoS2_MR | His-tagged CsoS2 Middle Region (MR), pET-14b backbone |
| 140844 | pBz110_CsoS2_CTD | His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone |
| 140845 | pLz47_polyPro_CsoS2_Npeptide | Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone |
| 140846 | pLz55_polyPro_random_Npeptide | Same as pLz47 but with randomized N*-peptide sequence |
| 140847 | pLz54_polyPro_polar | Same as pLz47 but with 20 polar amino acids instead of N*- peptide |
| 140848 | pLz56_polyPro_CsoS2_MR_r1 | Same as pLz47 but with MR repeat 1 instead of N*-peptide |
| 140849 | pLz57_polyPro_CsoS2_CTD_r8 | Same as pLz47 but with CTD repeat 8 instead of N*-peptide |
| 140850 | pLz58_polyPro_CsoS2_CTP | Same as pLz47 but with C-terminal peptide (CTP) instead of N*- peptide |
| 140851 | pLz26_CsoS2_N1-N2_GFP | His-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backbone |
| 140852 | pBz172_CsoS2_N1_GFP | His-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backbone |
| 140853 | pBz173_CsoS2_N2_GFP | His-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backbone |
| 140854 | pBz174_CsoS2_N3_GFP | His-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backbone |
| 140855 | pBz175_CsoS2_N4_GFP | His-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backbone |
| 140856 | pLz37_CB_operon_CsoS2_N1-N4 | Halothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli. |
| 140857 | pLz75_CB_operon_CsoS2_N3-N4 | Same as pLz37 but with CsoS2 truncated such that NTD repeats N1 and N2 are removed |
| 140858 | pLz76_CB_operon_CsoS2_N4 | Same as pLz37 but with CsoS2 truncated such that NTD repeats N1, N2, and N3 are removed |
| 140859 | pLz77_CB_operon_CsoS2_N3 | Same as pLz37 but with CsoS2 discontinuously truncated such that repeats N1, N2, and N4 are removed |
| 140860 | pLz78_CB_operon_CsoS2_delNTD | Same as pLz37 but with CsoS2 truncated to remove the entire NTD (i.e. N1, N2, N3, and N4 are removed) |
| 140861 | pLz33_CsoS2_NTD_GFP | His-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFP |