SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation.

Ros O, Baudet S, Zagar Y, Loulier K, Roche F, Couvet S, Aghaie A, Atkins M, Louail A, Petit C, Metin C, Mechulam Y, Nicol X
Cell Rep. 2020 Jul 21;32(3):107934. doi: 10.1016/j.celrep.2020.107934. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
140836	pCX-SpiCee	mammalian expression of SpiCee, a buffer of calcium that prevents downstream signaling
140837	pCX-Lyn-SpiCee	mammalian expression of Lyn-SpiCee, a lipid raft-targeted variant of the calcium buffer SpiCee that prevents downstream signaling
140838	pCX-SpiCee-Kras	mammalian expression of SpiCee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the calcium buffer SpiCee that prevents downstream signaling
140900	pCX-SpiCee-NLS	mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling
140901	pCX-SpiCee-NES	mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling
140902	pCX-NTOM-SpiCee	mammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signaling
140903	pCX-mutSpiCee	mammalian expression of a mutated form of SpiCee, that does not affect calcium signaling