AIMTOR, a BRET biosensor for live imaging, reveals subcellular mTOR signaling and dysfunctions.
Bouquier N, Moutin E, Tintignac LA, Reverbel A, Jublanc E, Sinnreich M, Chastagnier Y, Averous J, Fafournoux P, Verpelli C, Boeckers T, Carnac G, Perroy J, Ollendorff V
BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1186/s12915-020-00790-8.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140828 | AIMTOR T757 | AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells |
| 140829 | AIMTOR A757 | AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757 |