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Chromosomal barcoding of E. coli populations reveals lineage diversity dynamics at high resolution.

Jasinska W, Manhart M, Lerner J, Gauthier L, Serohijos AWR, Bershtein S
Nat Ecol Evol. 2020 Feb 24. pii: 10.1038/s41559-020-1103-z. doi: 10.1038/s41559-020-1103-z. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
141161Tn7 helper plasmidTemperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter
154134Tn7 integration plasmidSuicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.

Antibodies from Article