Multicopy chromosomal integration using CRISPR-associated transposases.
Zhang Y, Sun X, Wang Q, Xu J, Dong F, Yang S, Yang J, Zhang Z, Qian Y, Chen J, Zhang J, Liu Y, Tao R, Jiang Y, Yang J, Yang S
ACS Synth Biol. 2020 Jun 17. doi: 10.1021/acssynbio.0c00073.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140622 | pQCascade | Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNA |
| 140623 | pQCascade-array1 | Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-array1. The crRNA-array1 targets 8 different loci in Escherchia coli. |
| 140624 | pQCascade-IS1 | Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS1. The crRNA-IS1 targets IS1 loci in Escherchia coli. |
| 140625 | pQCascade-IS2 | Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS2. The crRNA-IS2 targets IS2 loci in Escherchia coli. |
| 140626 | pQCascade-IS3 | Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS3. The crRNA-IS3 targets IS3 loci in Escherchia coli. |
| 140627 | pQCascade-IS186 | Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli. |
| 140628 | pQCascade-tra13 | Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-tra13. The crRNA-tra13 targets 13 copies transposon loci in Tatumella citrea. |
| 140629 | pDonor-GDH | Encodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene. |
| 140630 | pSC101-Donor | Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication. |
| 140631 | pHelper-T7IS1 | Expresses ShCAST under the T7 promoter and an empty sgRNA |
| 140632 | pCutamp | Plasmid-curing in Escherichia coli by targeting the AmpR promoter |
| 160031 | pTnsABC | Expresses V. cholerae TnsABC |