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Highly Parallel Profiling of Cas9 Variant Specificity.

Schmid-Burgk JL, Gao L, Li D, Gardner Z, Strecker J, Lash B, Zhang F
Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
140560pX165-Sniper-Cas9Expresses Sniper Cas9 in mammalian cells
140561pX165-LZ3 Cas9Expresses LZ3 Cas9 in mammalian cells
140563pX165-HiFi Cas9Expresses HiFi Cas9 in mammalian cells
140564pX165-eSpCas9Expresses eSpCas9 in mammalian cells
140565pX165-Cas9-HF1Expresses Cas9-HF1 in mammalian cells
140567pX165-HypaCas9Expresses HypaCas9 in mammalian cells
140568pX165-xCas9Expresses xCas9 in mammalian cells
140569pX165-evoCas9Expresses evoCas9 in mammalian cells
140577pU6-pegRNA-HEK3+24Expresses a pegRNA in mammalian cells
140578pU6-pegRNA-DNMT1+38Expresses a pegRNA in mammalian cells
140579pU6-pegRNA-EMX1+38Expresses a pegRNA in mammalian cells
140580pmCherry-U6-emptyExpresses a gRNA and mCherry in mammalian cells
140581pmCherry-U6-EMX1Expresses a gRNA and mCherry in mammalian cells
140582pmCherry-U6-TTLL11Expresses a gRNA and mCherry in mammalian cells
140583pmCherry-U6-CLIC3Expresses a gRNA and mCherry in mammalian cells
140584pmCherry-U6-RNF103-CHMP3Expresses a gRNA and mCherry in mammalian cells
140585pmCherry-U6-RGS8Expresses a gRNA and mCherry in mammalian cells
140586pmCherry-U6-GTPBP2Expresses a gRNA and mCherry in mammalian cells
140587pmCherry-U6-SYNPOExpresses a gRNA and mCherry in mammalian cells
140588pmCherry-U6-VEGFAExpresses a gRNA and mCherry in mammalian cells
140589pmCherry-U6-ALDH1A3Expresses a gRNA and mCherry in mammalian cells
140590pmCherry-U6-CACNG3Expresses a gRNA and mCherry in mammalian cells

Antibodies from Article