Precise and broad scope genome editing based on high-specificity Cas9 nickases.
Wang Q, Liu J, Janssen JM, Le Bouteiller M, Frock RL, Goncalves MAFV
Nucleic Acids Res. 2021 Jan 25;49(2):1173-1198. doi: 10.1093/nar/gkaa1236.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199251 | BA31_pU.CAG.SaCas9-D10A.rBGpA | Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A) |
| 199252 | AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS | Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase |
| 199253 | AP76_pU.CAG.Cas9-D10A-K848A.rBGpA | Expression construct encoding the high specificity SpCas9-KA-D10A nickase |
| 199254 | AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA | Expression construct encoding the high specificity SpCas9-KARA-D10A nickase |
| 199256 | AB65_pCAG.Cas9-D10A.rBGpA | Expression construct encoding the SpCas9-D10A nickase |
| 199258 | AV44_pCAG.Cas9-D10A.gRNA.S1 | Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA |
| 199259 | AV15_pCAG.Cas9.gRNA.S1 | Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA |
| 199260 | AV13_pCAG.Cas9.gRNA.NT | Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA |