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Nessys: A new set of tools for the automated detection of nuclei within intact tissues and dense 3D cultures.

Blin G, Sadurska D, Portero Migueles R, Chen N, Watson JA, Lowell S
PLoS Biol. 2019 Aug 9;17(8):e3000388. doi: 10.1371/journal.pbio.3000388. eCollection 2019 Aug. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
183616pPyCAG-NE-mKate2-IRES-HphMammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered mKate2 (NLS-mKate2-EmerinTMD). Confers hygromycin B resistance.

Antibodies from Article