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Addgene

Standard screening methods underreport AAV-mediated transduction and gene editing.

Lang JF, Toulmin SA, Brida KL, Eisenlohr LC, Davidson BL
Nat Commun. 2019 Jul 30;10(1):3415. doi: 10.1038/s41467-019-11321-7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
174703pFB_ROSA26_SpCas9_gRNAsPlasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.

Antibodies from Article