Plug-and-Play Protein Modification Using Homology-Independent Universal Genome Engineering.
Gao Y, Hisey E, Bradshaw TWA, Erata E, Brown WE, Courtland JL, Uezu A, Xiang Y, Diao Y, Soderling SH
Neuron. 2019 Aug 21;103(4):583-597.e8. doi: 10.1016/j.neuron.2019.05.047. Epub 2019 Jul 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200368 | pHiUGE GS-gRNA (hSyn-Cre) backbone | Backbone plasmid for cloning HiUGE GS-gRNA (with hSyn-Cre) |
| 200369 | pHiUGE GS-gRNA (hSyn-Cre) mTubb3 | Positive control plasmid expressing mouse Tubb3 GS-gRNA (with hSyn-Cre) |
| 200370 | pHiUGE GS-gRNA (no Cre) | Backbone plasmid for cloning HiUGE GS-gRNA (without Cre) |
| 200371 | pHiUGE HA-epitope donor ORF0 | Tagging endogenously expressed proteins with HA epitope, C-terminal |
| 200372 | pHiUGE HA-epitope donor ORF1 | Tagging endogenously expressed proteins with HA epitope, C-terminal |
| 200373 | pHiUGE HA-epitope donor ORF2 | Tagging endogenously expressed proteins with HA epitope, C-terminal |
| 200374 | pHiUGE Myc-epitope donor ORF0 | Tagging endogenously expressed proteins with Myc epitope, C-terminal |
| 200375 | pHiUGE Myc-epitope donor ORF1 | Tagging endogenously expressed proteins with Myc epitope, C-terminal |
| 200376 | pHiUGE Myc-epitope donor ORF2 | Tagging endogenously expressed proteins with Myc epitope, C-terminal |
| 200377 | pHiUGE V5-epitope donor ORF0 | Tagging endogenously expressed proteins with V5 epitope, C-terminal |
| 200378 | pHiUGE V5-epitope donor ORF1 | Tagging endogenously expressed proteins with V5 epitope, C-terminal |
| 200379 | pHiUGE V5-epitope donor ORF2 | Tagging endogenously expressed proteins with V5 epitope, C-terminal |
| 200380 | pHiUGE smFP-V5 donor ORF0 | Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal |
| 200381 | pHiUGE smFP-V5 donor ORF1 | Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal |
| 200382 | pHiUGE smFP-V5 donor ORF2 | Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal |