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Thrombospondin-1 Mediates Axon Regeneration in Retinal Ganglion Cells.

Bray ER, Yungher BJ, Levay K, Ribeiro M, Dvoryanchikov G, Ayupe AC, Thakor K, Marks V, Randolph M, Danzi MC, Schmidt TM, Chaudhari N, Lemmon VP, Hattar S, Park KK
Neuron. 2019 Aug 21;103(4):642-657.e7. doi: 10.1016/j.neuron.2019.05.044. Epub 2019 Jun 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
154847pAAV.CMV.RGS4.C-HAExpresses murine RGS4 protein with HA-tag at C-terminus
154848pAAV.CMV.CD86.C-HAExpresses murine CD86 protein (T-lymphocyte activation antigen) with HA-tag at C-terminus
154849pAAV.CMV.VTN.C-HAExpresses murine Vitronectin (VTN) protein with HA-tag at C-terminus
154850pAAV.CMV.SV40.THBS1-HA.SV40(polyA)Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus
155193pAAV.CMV.SV40.THBS4-HA.SV40(polyA)Expresses murine Thrombospondin-4 (THBS4) protein with HA-tag at C-terminus
155194pAAV.CMV.SV40.THBS1-deltaTSR3-CTD-HA.SV40(polyA)Expresses truncated (AA 1-690) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus
155195pAAV.CMV.SV40.THBS1-deltaCC-HA.SV40(polyA)Expresses murine Thrombospondin-1 (THBS1) protein with deletion of Coiled Coil domain and HA-tag at C-terminus
156162pAAV.CMV.SV40.THBS1-deltaTSR1-HA.SV40(polyA)Expresses murine Thrombospondin-1 (THBS1) with deletion (AA 383-548) and HA-tag at C-terminus
156163pAAV.CMV.SV40.THBS1-deltaCTD-HA.SV40(polyA)Expresses truncated (AA 1-954) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus
156164pAAV.CMV.SV40.THBS1-HA-KDEL.SV40(polyA)Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus and KDEL (ER retention) sequence
195551pAAV-Bcl2Expresses Bcl2 in mammalian cells
195552pAAV.GFAP.SV40.Thbs1-HA.SV40(polyA)Expresses Thbs1 under control of short GFAP promoter
195558pAAV.GFAP.SV40.eGFP.SV40(polyA)Expresses eGFP under control of short GFAP promoter
195574pAAV.U6.shRNA-Sdc1-1376Expresses shRNA to knockdown mouse Syndecan-1

Antibodies from Article