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Cyclin F Controls Cell-Cycle Transcriptional Outputs by Directing the Degradation of the Three Activator E2Fs.

Clijsters L, Hoencamp C, Calis JJA, Marzio A, Handgraaf SM, Cuitino MC, Rosenberg BR, Leone G, Pagano M
Mol Cell. 2019 Jun 20;74(6):1264-1277.e7. doi: 10.1016/j.molcel.2019.04.010. Epub 2019 May 23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
236431pcDNA3 HA E2F1transient overexpression of E2F1 in mammalian cells
236432pcDNA3 HA E2F2transient overexpression of E2F2 in mammalian cells
236433pTRIPZ-2xFLAG-2xSTREP-E2F1doxycycline inducible expression of E2F1 in mammalian cells
236434pTRIPZ-2xFLAG-2xSTREP-E2F2doxycycline inducible expression of E2F2 in mammalian cells
236435pTRIPZ-2xFLAG-2xSTREP-E2F3Adoxycycline inducible expression of E2F3A in mammalian cells
236436pBABE-puro-2xFLAG-2xSTREP-E2F1stable expression of E2F1 using virus infection
236437pcDNA3 HA E2F3Atransient overexpression of E2F3A in mammalian cells
236438pcDNA3 HA E2F3Btransient overexpression of E2F3B in mammalian cells
236439pcDNA3 2xFLAG-2xSTREP E2F4transient overexpression of E2F4 in mammalian cells
236440pcDNA3 2xFLAG-2xSTREP E2F5transient overexpression of E2F5 in mammalian cells
236441pcDNA3 2xFLAG-2xSTREP E2F6transient overexpression of E2F6 in mammalian cells
236442pcDNA3 2xFLAG-2xSTREP E2F7transient overexpression of E2F7 in mammalian cells
236444pcDNA3 2xFLAG-2xSTREP TFDP1transient overexpression of TFDP1 in mammalian cells
236445pcDNA3 2xFLAG-2xSTREP TFDP2transient overexpression of TFDP2 in mammalian cells
236446NLS-Cherry-PCNA-P2A-Venus-E2F1insect expression of E2F1 for protein purification
236447NLS-Cherry-PCNA-P2A-Venus-E2F2insect expression of E2F2 for protein purification
236448NLS-Cherry-PCNA-P2A-Venus-E2F3insect expression of E2F3 for protein purification

Antibodies from Article