Anillin Promotes Cell Contractility by Cyclic Resetting of RhoA Residence Kinetics
Budnar S, Husain KB, Gomez GA, Naghibosadat M, Varma A, Verma S, Hamilton NA, Morris RG, Yap AS
Dev Cell. 2019 Jun 17;49(6):894-906.e12. doi: 10.1016/j.devcel.2019.04.031. Epub 2019 May 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187270 | pLL5.0-Anillin shRNA | Lentiviral expression of shRNA targeting Anillin |
| 187271 | PLL5.0-Anillin ShRNA-Cherry-AnillinFL | Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length |
| 187272 | PLL5.0-Anillin ShRNA-GFP-AnillinFL | Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length |
| 187273 | PLL5.0-Anillin ShRNA-Cherry-AnillinA740D, E758K | Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K |
| 187274 | PLL5.0-Anillin ShRNA-GFP-AnillinA740D, E758K | Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K |
| 187275 | PLL5.0-Anillin ShRNA-Cherry-Anillin deltaMyosin binding domain | Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Myosin binding domain |
| 187276 | PLL5.0-Anillin ShRNA-Cherry-Anillin deltaActin binding domain | Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Actin binding domain |
| 187277 | PLL5.0-Anillin ShRNA-Cherry-Anillin deltaAnillin homology domain | Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Anillin homology domain |
| 187279 | GFP-Rock1-GBD (840-110 aa) in pEGFPN1 | Express EGFP-Rock1 GTPase-binding domain |
| 187280 | GFP-mDia1-GBD (1-305 aa) in pEGFPN1 | Express EGFP-mDia1 GTPase-binding domain |
| 187282 | mRFP-PA-GFP-RhoAQ63L | Express pmRFP-PAGFP-RhoA Q63L |
| 187284 | IL2-mCherry-AH in pmCherryN1 | Express IL2-AH-mCherry |
| 187285 | IL2-mCherry-AHA740D, E758K in pmCherryN1 | Express IL2-AH A740D, E758K-mCherry |
| 187286 | IL2-mCherry-rGBD in pmCherryN1 | Express IL2-Rhotekin GTPase-binding domain-mCherry |
| 187287 | IL2-AH-2A-Cherry-RhoAQ63L in pmCherryN1 | Express pmCherry-IL2-AH-2A-RhoA Q63L |
| 187288 | IL2-AHA740D, E758K-2A-Cherry-RhoAQ63L in pmCherryN1 | Express pmCherry-IL2-AH A740D, E758K-2A-RhoA Q63L |
| 187292 | IL2-AHDC2-2A-CherryRhoAQ63L in pmCherryN1 | Express pmCherry-IL2-AH-deltaC2-2A-RhoA Q63L |