The 26S Proteasome Utilizes a Kinetic Gateway to Prioritize Substrate Degradation.
Bard JAM, Bashore C, Dong KC, Martin A
Cell. 2019 Apr 4;177(2):286-298.e15. doi: 10.1016/j.cell.2019.02.031. Epub 2019 Mar 28.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226680 | pAM88 | Rpt1I191TAG base (3xFLAG-Rpt1I191TAG, Rpt2, 6xHis-Rpt3, Rpt4, Rpt5, Rpt6) |
| 226681 | pAM89 | Rpt5Q49TAG base (3xFLAG-Rpt1, Rpt2, 6xHis-Rpt3, Rpt4, Rpt5Q49TAG, Rpt6) |
| 226682 | pAM90 | Rpn9S111TAG lid (Rpn5, MBP-HRV-Rpn6, Rpn8, Rpn9S111TAG, Rpn11) |
| 226686 | pAM94 | GGG-TitinV13PV15P-23-K-9-C-25-intein-CBD |
| 226687 | pAM95 | GGG-TitinV15P-43-K-8-C-25-intein-CBD |
| 226689 | pAM97 | GGG-Titinv15P-23-K-9-K-9-K-8-C-25 |
| 226690 | pAM98 | TitinV15P-23-K-9-C-15 |
| 226691 | pAM99 | TitinV15P-23-K-9-C-1 |
| 226692 | pAM100 | TitinV15P-21-C-2-K-1 |
| 226694 | pAM102 | 6xHis-Sumo-Ub-Ub-Ub-Ub |
| 226696 | pAM104 | GGG-TitinV15P-43-K-11-intein-CBD |
| 226697 | pAM105 | GGG-TitinV15P-23-K-19-K-11-intein-CBD |