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Cancer Mutations of the Tumor Suppressor SPOP Disrupt the Formation of Active, Phase-Separated Compartments.

Bouchard JJ, Otero JH, Scott DC, Szulc E, Martin EW, Sabri N, Granata D, Marzahn MR, Lindorff-Larsen K, Salvatella X, Schulman BA, Mittag T
Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi: 10.1016/j.molcel.2018.08.027. Epub 2018 Sep 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
169720SPOP-V5 (WT)Mammalian Expression of wild-type mouse SPOP
169721SPOP-V5 (BTB)Mammalian Expression of mouse SPOP-BTB (L186D, L190D, L193D, I217K)
169722SPOP-V5 (BACK))Mammalian Expression of mouse SPOP Y353E
169723SPOP-V5 (BTB+BACK)Mammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)
169724SPOP-V5 (W131G)Mammalian Expression of mouse SPOP W131G
169725SPOP-V5 (F133V)Mammalian Expression of mouse SPOP F133V
169726pcDNA6.2EmGFP-DAXXMammalian expression construct of GFP tagged full length DAXX
169727pcDNA6.2EmGFP-cDAXXMammalian expression construct of GFP tagged cDAXX
169728pcDNA6.2EmGFP-cDAXX-0SBMammalian expression construct of GFP tagged cDAXX-0SB
169729pDEST17-cDAXXBacterial expression of N-terminally 6His tagged cDAXX
169730pDEST17-cDAXX-0SBBacterial expression of N-terminally 6His tagged cDAXX-0SB

Antibodies from Article