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Genomic Profiling by ALaP-Seq Reveals Transcriptional Regulation by PML Bodies through DNMT3A Exclusion.

Kurihara M, Kato K, Sanbo C, Shigenobu S, Ohkawa Y, Fuchigami T, Miyanari Y
Mol Cell. 2020 May 7;78(3):493-505.e8. doi: 10.1016/j.molcel.2020.04.004. Epub 2020 Apr 29. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
140273pBlue-loxPuro-APEX-PML_LASAa targeting plasmid for APEX-PML
140279pGbH500-Nanog-AXPa targeting plasmid for Nanog-APEX
140280pX300A-G+NanogCRISPR/Cas9 plasmid encoding Cas9 and sgRNA against gBait and Nanog locus
140281pEF-APEX2-NLSExpression of APEX2-NLS
140283pGb_PMLlxP_EGFPa targeting plasmid for EGFP-PML
140284pCAG-Cre-iBSDExpression of Cre-IRES-BSD
140285pYMCR2_Gb_PMLTSSCRISPR/Cas9 plasmids encoding sgRNA targeting TSS of PML for knock-in
140286pCFL-mPML-2CAMut-iBExpression of mouse PML Ca mutant
140287pCFL-mPML-3KRmut-iBExpression of mouse PML KR mutant

Antibodies from Article