Orthogonal Protein-Responsive mRNA Switches for Mammalian Synthetic Biology.
Ono H, Kawasaki S, Saito H
ACS Synth Biol. 2020 Jan 17;9(1):169-174. doi: 10.1021/acssynbio.9b00343. Epub 2019 Dec 13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140258 | pscMS2(WTx2)-EGFP | MS2CP-dependent translational repression |
| 140259 | pMS2CP-myc-T2A-tagRFP | Expresses WT MS2CP in mammalian cells |
| 140260 | pMS2CP(V29I)-myc-T2A-tagRFP | Expresses MS2CP(V29I) in mammalian cells |
| 140261 | pscPP7(2xWT)-EGFP | PP7CP-dependent translational repression |
| 140262 | pPP7CP-myc-T2A-tagRFP | Expresses PP7CP in mammalian cells |
| 140263 | pL7Ae-myc-T2A-tagRFP | Expresses L7Ae in mammalian cells |
| 140264 | pU1A_full-myc-T2A-tagRFP | Expresses U1A in mammalian cells |