CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells.
Kurt IC, Zhou R, Iyer S, Garcia SP, Miller BR, Langner LM, Grunewald J, Joung JK
Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140251 | SECURE BE4max(R33A) (pBM608) | CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP |
| 140252 | CGBE1 (pRZ3885) | CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP |
| 140253 | miniCGBE1 (pBM1219) | CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP |
| 140254 | CGBE1-VRQR (pBM1192) | CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP |
| 140255 | miniCGBE1-VRQR (pBM1173) | CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP |
| 140256 | CGBE1-NG (pBM1246) | CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP |
| 140257 | miniCGBE1-NG (pBM1182) | CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP |
| 170105 | miniCGBE1-SpRY (pBM1571) | CMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP |