An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue.
Jin J, Xu Y, Huo L, Ma L, Scott AW, Pizzi MP, Li Y, Wang Y, Yao X, Song S, Ajani JA
PLoS One. 2020 Feb 13;15(2):e0228910. doi: 10.1371/journal.pone.0228910. eCollection 2020.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140206 | pLentiCrispr-V2-mOrange (V2mO) | Lenti Crispr/Cas9 with mOrange fluorescent marker |