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The chloroalkaloid (-)-acutumine is biosynthesized via a Fe(II)- and 2-oxoglutarate-dependent halogenase in Menispermaceae plants.

Kim CY, Mitchell AJ, Glinkerman CM, Li FS, Pluskal T, Weng JK
Nat Commun. 2020 Apr 20;11(1):1867. doi: 10.1038/s41467-020-15777-w. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
140128pHis8-4b-SaDAHExpresses SaDAH with N-terminal 8xHis-tag in BL21 E. coli
140129pHis8-4b-McDAHExpresses McDAH with N-terminal 8xHis-tag in BL21 E. coli
140130pHIs8-4b-SaDAH::G226DExpresses SaDAH::G226D variant with N-terminal 8xHis-tag in BL21 E. coli

Antibodies from Article