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A fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity enables live imaging of infection in single cells and viral plaques.

Arias-Arias JL, MacPherson DJ, Hill ME, Hardy JA, Mora-Rodriguez R
J Biol Chem. 2020 Jan 9. pii: RA119.011319. doi: 10.1074/jbc.RA119.011319. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
140088pLenti-FlaviA-GFP-puroFluorescence activatable reporter of flavivirus NS2B-NS3 protease activity
140089pLenti-DENV2A-GFP-puroFluorescence activatable reporter of DENV-2 NS2B-NS3 protease activity
140090pLenti-ZIKVA-GFP-puroFluorescence activatable reporter of ZIKV NS2B-NS3 protease activity
140091pLenti-CMV-FlaviA-mNeptune-puroFluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

Antibodies from Article