Multiple Input Sensing and Signal Integration Using a Split Cas12a System.
Kempton HR, Goudy LE, Love KS, Qi LS
Mol Cell. 2020 Jan 30. pii: S1097-2765(20)30037-X. doi: 10.1016/j.molcel.2020.01.016.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140086 | pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE | Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance |
| 140087 | pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPRE | Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistance |
| 140220 | pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA | Constitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activator |
| 140221 | pSLQ8518 PB-PGK-N term (1-406) dLbCpf1-EE12RR345L-pA | Constitutive PGK driven expression of N terminal dCpf1 (Cas12a) 406-split half with leucine zipper |
| 140222 | pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA | Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR |
| 140223 | pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA | Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activator |
| 140224 | pSLQ8637 PB-PGK-C intein-2XNLS-miniVPR-mCherry-pA | Constitutive PGK driven expression of intein with miniVPR for 3-way split activator |
| 140226 | pSLQ8611 PB-Tre3g-Fluc-DR-crIFNg-DR-crIFNg-DR-SV40PolyA/ R(EF1a- Zeo–t2A-rtTA-WPRE) | Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and zeocin resistance |
| 140227 | pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-EFS-Puro-WPRE | Hypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance. |
| 140228 | pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE | RRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance. |
| 140229 | pSLQ8702 pHR-TRE3G-AcrVA1-EF1a-sfGFP-T2A-rtta-WPRE | Dox-inducible expression of anti-Cpf1 AcrVA1. Constitutive EF1a driven rtTA and GFP |
| 140230 | pSLQ8703 pHR-R(TRE3G-Fluc-2XcrIFNg-SV40pA)-EF1a-sfGFP-T2A-rtta-WPRE | Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and GFP |