Time-resolved imaging-based CRISPRi screening.
Camsund D, Lawson MJ, Larsson J, Jones D, Zikrin S, Fange D, Elf J
Nat Methods. 2020 Jan;17(1):86-92. doi: 10.1038/s41592-019-0629-y. Epub 2019 Nov 18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140040 | pGuide-P1-O1a (EL702) | CRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs. |