Addgene: Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.

Walton RT, Christie KA, Whittaker MN, Kleinstiver BP
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
140005	pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)	CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
140004	pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)	CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
140003	pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)	CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
140002	pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)	CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
140001	pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)	CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
140000	pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)	CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
139999	pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)	CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
139998	pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)	CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
139997	pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)	CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
139996	pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)	CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Antibodies from Article