Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Walton RT, Christie KA, Whittaker MN, Kleinstiver BP
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 139987 | pCMV-T7-SpCas9-P2A-EGFP (RTW3027) | CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP |
| 139988 | pCMV-T7-SpG-P2A-EGFP (RTW4177) | CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP |
| 139989 | pCMV-T7-SpRY-P2A-EGFP (RTW4830) | CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP |
| 139990 | pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520) | CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP |
| 139991 | pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160) | CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP |
| 139992 | pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161) | CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP |
| 139993 | pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576) | CMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP |
| 139994 | pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677) | CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP |
| 139995 | pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505) | CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP |
| 139996 | pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000) | CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP |
| 139997 | pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008) | CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP |
| 139998 | pCAG-CBE4max-SpG-P2A-EGFP (RTW4552) | CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP |
| 139999 | pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133) | CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP |
| 140000 | pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637) | CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP |
| 140001 | pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554) | CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP |
| 140002 | pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) | CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP |
| 140003 | pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) | CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP |
| 140004 | pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644) | CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP |
| 140005 | pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564) | CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP |