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An engineered enzyme that targets circulating lactate to alleviate intracellular NADH:NAD(+) imbalance.

Patgiri A, Skinner OS, Miyazaki Y, Schleifer G, Marutani E, Shah H, Sharma R, Goodman RP, To TL, Robert Bao X, Ichinose F, Zapol WM, Mootha VK
Nat Biotechnol. 2020 Jan 13. pii: 10.1038/s41587-019-0377-7. doi: 10.1038/s41587-019-0377-7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
139971pET30a-His6-ABP-LOXCATFusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).
139972pET30a-His6-ABP-LOXCATmutA control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)
140084pET30a-His6-LOXCATFusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.
140085pET30a-His6-LOXCATmutA control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

Antibodies from Article