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A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.

Liang JR, Lingeman E, Luong T, Ahmed S, Muhar M, Nguyen T, Olzmann JA, Corn JE
Cell. 2020 Mar 19;180(6):1160-1177.e20. doi: 10.1016/j.cell.2020.02.017. Epub 2020 Mar 10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
139840pLenti-X1-Neo-NDUFB4Lentiviral expression of human NDUFB4
139841pLenti-X1-Neo-NDUFB2Lentiviral expression of human NDUFB2
139842pLenti-X1-Neo-ATP5OLentiviral expression of human ATP5O
139843pLenti-XI-Neo-GST-Constitutively Active AMPKLentiviral expression of GST-tagged Constitutively Active rat AMPK
139844pLenti-XI-Neo-GST- AMPK- Kinase Dead (K to R)Lentiviral expression of GST-tagged rat AMPK- Kinase Dead (K to R)
139845pLenti-X1-Neo-DDRGK1-WT-HALentiviral expression of human DDRGK1-WT-HA
139846pLenti-X1-Neo-DDRGK1-dTM-HALentiviral expression of human DDRGK1-dTM-HA
139847pLenti-X1-Neo-DDRGK1-dPCI-HALentiviral expression of human DDRGK1-dPCI-HA
139848pLenti-X1-Neo-DDRGK1-K267R-HALentiviral expression of human DDRGK1-K267R-HA
139849pLenti-X1-Neo-DDRGK1-K-less-HALentiviral expression of human DDRGK1-K-less-HA
139850pLenti-X1-Neo-DDRGK1-K116R-HALentiviral expression of human DDRGK1-K116R-HA
139851pLenti-X1-Neo-DDRGK1-K120R-HALentiviral expression of human DDRGK1-K120R-HA
139852pLenti-X1-Neo-DDRGK1-K121R-HALentiviral expression of the cDNA indicated
139853pLenti-X1-Neo-DDRGK1-K124R-HALentiviral expression of human DDRGK1-K124R-HA
139854pLenti-X1-Neo-DDRGK1-K128R-HALentiviral expression of human DDRGK1-K128R-HA
139855pLenti-X1-Neo-DDRGK1-K146R-HALentiviral expression of human DDRGK1-K146R-HA
139856pLenti-X1-Neo-DDRGK1-K176R-HALentiviral expression of human DDRGK1-K176R-HA
139857pLenti-X1-Neo-DDRGK1-K193R-HALentiviral expression of the cDNA indicated
139858pLenti-X1-Neo-DDRGK1-K224R-HALentiviral expression of human DDRGK1-K224R-HA
139859pLenti-X1-Neo-DDRGK1-K227R-HALentiviral expression of human DDRGK1-K227R-HA
139860pLenti-X1-Neo-HA-UFL1Lentiviral expression of HA-tagged human UFL1
139861pLenti-X1-Neo-TOM20MTS-DDRGK1-dTM-HA Lentiviral expression of TOM20MTS-DDRGK1-dTM-HA
139862pLenti-X1-Neo-PMP34-DDRGK1-dTM-HA Lentiviral expression of PMP34-DDRGK1-dTM-HA
139863pLenti-X1-Neo-HA-UFL1-RAMP4Lentiviral expression of fusion of human UFL1 (full length) with RAMP4
139865pLenti-X1-Neo-HA-SEC62Lentiviral expression of HA-tagged human SEC62
139866pLenti-X1-Neo-TEX264-HALentiviral expression of HA tagged (C terminal) human TEX264
139867pLenti-X1-Neo-HA-CCPG1Lentiviral expression of HA-tagged human CCPG1
139868plenti-X1-Neo-RPN1-HA Lentiviral expression of HA-tagged (C terminal) human RPN1
139869pRK5-HA-UFM1-dCSMammalian expression of HA-tagged human UFM1-cCS
139870TetOn-REEP5-mCherry-eGFPDox-inducible lentiviral expression of human REEP5
139871TetOn-CLIMP63-mCherry-eGFPDox-inducible lentiviral expression of human CLIMP63

Antibodies from Article