Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice.
Ali Z, Shami A, Sedeek K, Kamel R, Alhabsi A, Tehseen M, Hassan N, Butt H, Kababji A, Hamdan SM, Mahfouz MM
Commun Biol. 2020 Jan 23;3(1):44. doi: 10.1038/s42003-020-0768-9.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
139802 | pRGEB-Cas9-VirD2 (PCO) | Expressing Cas9-VirD fusion under ubiquitin promoter |
139803 | pRGEB-VirD2-Cas9 | Expressing VirD-Cas9 fusion under ubiquitin promoter |