HR-Bac, a toolbox based on homologous recombination for expression, screening and production of multiprotein complexes using the baculovirus expression system.
Kolesnikova O, Zachayus A, Pichard S, Osz J, Rochel N, Rossolillo P, Kolb-Cheynel I, Troffer-Charlier N, Compe E, Bensaude O, Berger I, Poterszman A
Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.1038/s41598-021-04715-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 139768 | pAC8_MF (VE5596) | Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette. |
| 139769 | pAC8_MF-p10-mCh PH-mCh (VE5625) | Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an mCherry cDNA under each promoter. |
| 139770 | pAC8_MF-P10-mCherry-pH-3C-TwinStrep (VE5621) | Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter TwinStrep tag under the PH promoter. |
| 139771 | pAC8_MF-PH-3C-10His-Cter (VE5622) | Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter 10-His tag under the pH promoter. |
| 139772 | pAC8_MF-p10-mCh (VE5626) | Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with mCherry cDNA under p10 to monitor infection. |
| 139773 | pAC8_MF-PH-Flag-Nter (VE5627) | Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an N-ter Flag tag under the pH promoter. |
| 139775 | pAC8_MF-PH-HA-Cter (VE5629) | Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter HA tag under the pH promoter. |
| 139776 | pAC8_MF-p10-10His-Cter (VE5742) | Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the pH promoter. |
| 139777 | pAC8_MF-PH-6His-Cter (VE5630) | Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter6His tag under the pH promoter. |
| 139778 | pAC8_MF-PH-c-Myc-Cter (VE5632) | Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter C-Myc tag under the pH promoter. |
| 161798 | pAC8_MF-p10-Flag-Cter(VE5738) | Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the p10 promoter. |
| 161799 | pAC8_MF-pH-Flag-Cter (VE5739) | Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the pH promoter. |
| 161800 | pAC8_MF-PH-HA-Nter-P10-mCherry (VE5740) | Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a N-ter HA tag under the pH promoter. |
| 161802 | pAC8_MF-PH-10His-Cter (VE5631) | Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the p10 promoter. |
| 161806 | pAC8_PH-10His-Nter-GWs-Lox (VE5588) | Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 10 His tag under the pH promoter. |
| 163766 | pAC8_PH-GST-Nter-GWs-Lox (VE5586) | Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter GST tag under the pH promoter. |
| 163768 | pAC8_PH-6His-Nter-GWs-Lox (VE5587) | Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 6 His tag under the pH promoter. |
| 163769 | pAC8_GWs-Lox (VE5589) | Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette under the pH promoter. |