Single-particle imaging of stress-promoters induction reveals the interplay between MAPK signaling, chromatin and transcription factors.
Wosika V, Pelet S
Nat Commun. 2020 Jun 23;11(1):3171. doi: 10.1038/s41467-020-16943-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 139487 | pSP264 | Insertion of the pSTL1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae |
| 139488 | pSP266 | Insertion of the pCTT1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae |
| 139489 | pVW200 | Insertion of the pGPD1 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae |
| 139490 | pVW293 | Insertion of the pHSP12 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae |
| 139491 | pVW294 | Insertion of the pGRE2 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae |
| 139492 | pVW295 | Insertion of the pALD3 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae |
| 139493 | pSP568 | Insertion of the pSTL1 promoter and 24 MS2 stem loops in the GLT1 locus using HIS marker in S. cerevisiae |
| 139494 | pVW284 | Expression vector for the PP7-GFP protein in S. cerevisiae |
| 139495 | pVW300 | Expression vector for the PP7-GFP protein in S. cerevisiae |
| 139496 | pVW296 | Expression vector for the PP7-mCherry protein in S. cerevisiae |
| 139497 | pSP561 | Expression vector for the MS2-GFP protein in S. cerevisiae |
| 139498 | pSP571 | CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA |
| 139499 | pSP569 | Repair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiae |
| 139500 | pSP566 | Gene tagging vector to add mCherry Tag and membrane anchoring motif |